Western Blot (WB)
(ISWI Antibody (N-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the ISWI antibody detected the ISWI protein (arrow).)
Rabbit anti-Human ISWI Polyclonal Antibody | anti-Iswi antibody
ISWI, NT (SMARCA5, SNF2H, WCRF135, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5, Sucrose nonfermenting protein 2 homolog)
Gene Names
Iswi; ACF; anon-EP1279744.124; CG8625; CHRAC; dCHRAC; dISWI; DmelCG8625; dNURF; ISW; iswi; ISWI; NURF; Nurf-140; NURF-140; p140; p140/ISWI; SWI
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
ISWI; Polyclonal Antibody; NT (SMARCA5; SNF2H; WCRF135; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; Sucrose nonfermenting protein 2 homolog); Anti -ISWI; anti-Iswi antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-Iswi antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
ISWI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-91 amino acids from the N-terminal region of human ISWI.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(ISWI Antibody (N-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the ISWI antibody detected the ISWI protein (arrow).)
Western Blot (WB)
(ISWI Antibody (N-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the ISWI antibody detected the ISWI protein (arrow).)
Related Product Information for anti-Iswi antibody
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein.
Product Categories/Family for anti-Iswi antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
118,873 Da
NCBI Official Full Name
imitation SWI, isoform C
NCBI Official Synonym Full Names
Imitation SWI
NCBI Official Symbol
Iswi
NCBI Official Synonym Symbols
ACF; anon-EP1279744.124; CG8625; CHRAC; dCHRAC; dISWI; DmelCG8625; dNURF; ISW; iswi; ISWI; NURF; Nurf-140; NURF-140; p140; p140/ISWI; SWI
NCBI Protein Information
CG8625 gene product from transcript CG8625-RA; CG8625-PA; CG8625-PB; CG8625-PC; ISWI ATPase; Iswi-PA; Iswi-PB; Iswi-PC; imitation SW; imitation SWI; imitation switch; imitation-SWI; nucleosome remodeling factor; nucleosome remodeling factor - 140kD
UniProt Protein Name
Chromatin-remodeling complex ATPase chain Iswi
Protein Family
UniProt Gene Name
Iswi
UniProt Synonym Gene Names
NURF-140
UniProt Entry Name
ISWI_DROME
Uniprot Description
Function: Energy-transducing component of the chromatin-remodeling complexes NURF (nucleosome-remodeling factor), ACF (ATP-utilizing chromatin assembly and remodeling factor), and CHRAC (chromatin accessibility complex). NURF catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. It is required for homeotic gene expression, proper larval blood cell development, normal male X chromosome morphology, ecdysteroid signaling and metamorphosis. Ref.6 Ref.7 Ref.8 Ref.9
Subunit structure: Component of the NURF complex composed of Caf1, E(bx), Nurf-38 and Iswi. Ref.6
Subcellular location: Nucleus Ref.6.
Developmental stage: Present throughout embryonic, larval and pupal development and in female adults. Present at low levels in adult males (at protein level). Ref.6
Sequence similarities: Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.Contains 1 helicase ATP-binding domain.Contains 1 helicase C-terminal domain.Contains 2 SANT domains.
Research Articles on Iswi
Similar Products
Product Notes
The Iswi iswi (Catalog #AAA6009337) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ISWI, NT (SMARCA5, SNF2H, WCRF135, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5, Sucrose nonfermenting protein 2 homolog) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ISWI can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the Iswi iswi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ISWI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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