Rabbit anti-Human ISWI Polyclonal Antibody | anti-ISWI antibody

ISWI, CT (SMARCA5, SNF2H, WCRF135, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5, Sucrose nonfermenting protein 2 homolog) (MaxLight 490)

Cat. Num. AAA6311109

• Gene Names: SMARCA5; ISWI; SNF2H; hISWI; hSNF2H; WCRF135
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ISWI; Polyclonal Antibody; CT (SMARCA5; SNF2H; WCRF135; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; Sucrose nonfermenting protein 2 homolog) (MaxLight 490); SMARCA5; Sucrose nonfermenting protein 2 homolog; anti-ISWI antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ISWI.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
• Sequence Length: 1052

• Applicable Applications for anti-ISWI antibody: Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 946-975 in the C-terminal region of human ISWI, UniProt Accession #NP_003592.2;O60264; Q91ZW3. Species sequence homology: mouse
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS6008064. This demonstrates that MBS6008064 detected the ISWI protein (arrow).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of K562 cells using MBS6008064 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ISWI antibody

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein.

Product Categories/Family for anti-ISWI antibody

Antibodies; Actin Related Proteins

References

1. Goldman, J.A., et al. J. Biol. Chem. 285(7):4645-4651(2010). 2. He, X., et al. Biochemistry 47(27):7025-7033(2008). 3. Sheu, J.J., et al. Cancer Res. 68(11):4050-4057(2008). 4. Olsen, J.V., et al. Cell 127(3):635-648(2006). 5. Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 21071058
• NCBI GeneID: 8467
• NCBI Accession #: NP_003592.2
• NCBI GenBank Nucleotide #: NP_003592.2
• UniProt Accession #: O60264; Q91ZW3
• NCBI Official Full Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
• NCBI Official Synonym Full Names: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
• NCBI Official Symbol: SMARCA5
• NCBI Official Synonym Symbols: ISWI; SNF2H; hISWI; hSNF2H; WCRF135
• NCBI Protein Information: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
• UniProt Protein Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
• Protein Family: ISWI one complex protein
• UniProt Gene Name: SMARCA5
• UniProt Synonym Gene Names: SNF2H; WCRF135; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; hSNF2H
• UniProt Entry Name: SMCA5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008]