Rabbit anti-Human ISWI Polyclonal Antibody | anti-ISWI antibody

ISWI, NT (SMARCA5, SNF2H, WCRF135, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5, Sucrose nonfermenting protein 2 homolog) (FITC)

Cat. Num. AAA6311117

• Gene Names: SMARCA5; ISWI; SNF2H; hISWI; hSNF2H; WCRF135
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ISWI; Polyclonal Antibody; NT (SMARCA5; SNF2H; WCRF135; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; Sucrose nonfermenting protein 2 homolog) (FITC); SMARCA5; Sucrose nonfermenting protein 2 homolog; anti-ISWI antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 1052

• Applicable Applications for anti-ISWI antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ISWI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-91 amino acids from the N-terminal region of human ISWI.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ISWI Antibody (N-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the ISWI antibody detected the ISWI protein (arrow).)

Related Product Information for anti-ISWI antibody

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein.

Product Categories/Family for anti-ISWI antibody

Antibodies; Actin Related Proteins

References

Goldman, J.A., et al. J. Biol. Chem. 285(7):4645-4651(2010). He, X., et al. Biochemistry 47(27):7025-7033(2008). Sheu, J.J., et al. Cancer Res. 68(11):4050-4057(2008). Olsen, J.V., et al. Cell 127(3):635-648(2006). Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 21071058
• NCBI GeneID: 8467
• NCBI Accession #: NP_003592.2
• NCBI GenBank Nucleotide #: NP_003592.2
• UniProt Accession #: O60264
• NCBI Official Full Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
• NCBI Official Synonym Full Names: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
• NCBI Official Symbol: SMARCA5
• NCBI Official Synonym Symbols: ISWI; SNF2H; hISWI; hSNF2H; WCRF135
• NCBI Protein Information: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
• UniProt Protein Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
• Protein Family: ISWI one complex protein
• UniProt Gene Name: SMARCA5
• UniProt Synonym Gene Names: SNF2H; WCRF135; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; hSNF2H
• UniProt Entry Name: SMCA5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008]