Rat 14-3-3 protein zeta/delta (YWHAZ) ELISA Kit | YWHAZ elisa kit

Rat 14-3-3 protein zeta/delta (YWHAZ) ELISA Kit

Cat. Num. AAA7226302

• Gene Names: YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; 14-3-3-zeta
• Reactivity: Rat
• Synonyms: 14-3-3 protein zeta/delta (YWHAZ); Rat 14-3-3 protein zeta/delta (YWHAZ) ELISA Kit; YWHAZ elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 25-500 ng/mL
• Sensitivity: 1.0ng/ml
• Intended Uses: This YWHAZ ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat YWHAZ. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for YWHAZ elisa kit

Principle of the assay: YWHAZ ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-YWHAZ antibody and an YWHAZ-HRP conjugate. The assay sample and buffer are incubated together with YWHAZ-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YWHAZ concentration since YWHAZ from samples and YWHAZ-HRP conjugate compete for the anti-YWHAZ antibody binding site. Since the number of sites is limited, as more sites are occupied by YWHAZ from the sample, fewer sites are left to bind YWHAZ-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YWHAZ concentration in each sample is interpolated from this standard curve.

Product Categories/Family for YWHAZ elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4507953
• NCBI GeneID: 7534
• NCBI Accession #: NP_003397.1
• NCBI GenBank Nucleotide #: NM_003406.3
• Molecular Weight: 19,333 Da
• NCBI Official Full Name: 14-3-3 protein zeta/delta
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta
• NCBI Official Symbol: YWHAZ
• NCBI Official Synonym Symbols: HEL4; YWHAD; KCIP-1; HEL-S-3; 14-3-3-zeta
• NCBI Protein Information: 14-3-3 protein zeta/delta; 14-3-3 zeta; 14-3-3 delta; phospholipase A2; epididymis luminal protein 4; epididymis secretory protein Li 3; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; 14-3-3 protein/cytosolic phospholipase A2; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide
• UniProt Protein Name: 14-3-3 protein zeta/delta
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAZ
• UniProt Synonym Gene Names: KCIP-1
• UniProt Entry Name: 1433Z_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

14-3-3 zeta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Phosphorylation apparently disrupts homodimerization.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 8q23.1

Cellular Component: extracellular space; focal adhesion; cytoplasmic vesicle membrane; mast cell granule; protein complex; mitochondrion; postsynaptic density; leading edge; cytosol; nucleoplasm; perinuclear region of cytoplasm; cytoplasm; melanosome; nucleus

Molecular Function: identical protein binding; protein domain specific binding; protein binding; protein complex binding; transcription factor binding; protein kinase binding

Biological Process: response to drug; platelet activation; histamine secretion by mast cell; apoptosis; establishment of Golgi localization; gene expression; blood coagulation; signal transduction; protein targeting to mitochondrion; negative regulation of apoptosis