Rabbit anti-Human, Mouse ISL1 Polyclonal Antibody | anti-ISL1 antibody

ISL1, ID (ISL1, Insulin gene enhancer protein ISL-1) (FITC)

Cat. Num. AAA6311051

• Gene Names: ISL1; Isl-1; ISLET1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ISL1; Polyclonal Antibody; ID (ISL1; Insulin gene enhancer protein ISL-1) (FITC); Insulin gene enhancer protein ISL-1; anti-ISL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ISL1. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ISL1 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 156-185 in the central region of human ISL1, UniProt Accession #P61371. Species sequence homology: chicken, zebrafish and rat
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB231 cell line lysates (35ug/lane) usingMBS6009105. This demonstrates that MBS6009105 detected the ISL1 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse bladder tissue lysates (35ug/lane) usingMBS6009105. This demonstrates that MBS6009105 detected the ISL1 protein (arrow).)

Related Product Information for anti-ISL1 antibody

ISL1 is a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Product Categories/Family for anti-ISL1 antibody

Antibodies; Insulin

References

1. De Luca, A., et al. Clin. Genet. (2010). In press 2. Davis, O.S., et al. Behav. Genet. (2010). In press 3. Genead, R., et al. Stem Cell Res 4(1):69-76(2010). 4. Stevens, K.N., et al. PLoS ONE 5 (5), E10855 (2010). 5. Zhang, H., et al. J. Mol. Biol. 392(3):566-577(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 115387114
• NCBI GeneID: 3670
• NCBI Accession #: NP_002193.2
• NCBI GenBank Nucleotide #: NM_002202.2
• UniProt Accession #: P61371; P20663; P47894
• Molecular Weight: 39,036 Da
• NCBI Official Full Name: insulin gene enhancer protein ISL-1
• NCBI Official Synonym Full Names: ISL LIM homeobox 1
• NCBI Official Symbol: ISL1
• NCBI Official Synonym Symbols: Isl-1; ISLET1
• NCBI Protein Information: insulin gene enhancer protein ISL-1
• UniProt Protein Name: Insulin gene enhancer protein ISL-1
• Protein Family: Insulin gene enhancer protein
• UniProt Gene Name: ISL1
• UniProt Synonym Gene Names: Islet-1
• UniProt Entry Name: ISL1_HUMAN

NCBI Description

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq, Jul 2008]

Uniprot Description

ISL1: Binds to one of the cis-acting domain of the insulin gene enhancer.

Protein type: Cell development/differentiation; Nuclear receptor co-regulator; DNA-binding; Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q11.1

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus

Molecular Function: ligand-dependent nuclear receptor binding; zinc ion binding; bHLH transcription factor binding; estrogen receptor binding; chromatin binding

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of interleukin-12 production; positive regulation of interleukin-1 alpha production; negative regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of estrogen receptor signaling pathway; pancreas development; positive regulation of cell proliferation; ventricular cardiac muscle morphogenesis; neuron fate specification; negative regulation of neuron apoptosis; sensory system development; neural crest cell migration; positive regulation of DNA binding; trigeminal nerve development; pharyngeal system development; axon regeneration; spinal cord motor neuron cell fate specification; positive regulation of insulin secretion; positive regulation of interleukin-6 production; spinal cord motor neuron differentiation; positive regulation of tumor necrosis factor production; positive regulation of interleukin-1 beta production; positive regulation of interferon-gamma production; negative regulation of neuron differentiation; positive regulation of angiogenesis; mesenchymal cell differentiation; visceral motor neuron differentiation; pituitary gland development; negative regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; peripheral nervous system neuron axonogenesis; retinal ganglion cell axon guidance