Western Blot (WB)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 2ug/ml Rabbit Anti-Human HCK Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human Hemopoietic Cell Kinase Polyclonal Antibody | anti-HCK antibody
Polyclonal Antibody to Hemopoietic Cell Kinase (HCK)
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 2ug/ml Rabbit Anti-Human HCK Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 2ug/ml Rabbit Anti-Human HCK Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010]
Uniprot Description
Hck: a protein-tyrosine kinase of the Src family that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms that have different subcellular localization.
Protein type: EC 2.7.10.2; Kinase, protein; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Src family; TK group
Chromosomal Location of Human Ortholog: 20q11.21
Cellular Component: actin filament; caveola; cell projection; cytosol; extrinsic component of cytoplasmic side of plasma membrane; focal adhesion; Golgi apparatus; lysosome; nucleoplasm; plasma membrane; transport vesicle
Molecular Function: ATP binding; non-membrane spanning protein tyrosine kinase activity; phosphotyrosine binding; protein binding; protein tyrosine kinase activity; receptor binding
Biological Process: cell adhesion; cell differentiation; cytokine-mediated signaling pathway; Fc-gamma receptor signaling pathway involved in phagocytosis; inflammatory response; innate immune response-activating signal transduction; integrin-mediated signaling pathway; leukocyte degranulation; leukocyte migration during immune response; lipopolysaccharide-mediated signaling pathway; mesoderm development; negative regulation of apoptosis; peptidyl-tyrosine autophosphorylation; peptidyl-tyrosine phosphorylation; positive regulation of actin cytoskeleton reorganization; positive regulation of actin filament polymerization; positive regulation of cell proliferation; protein amino acid phosphorylation; protein autophosphorylation; regulation of cell shape; regulation of defense response to virus by virus; regulation of inflammatory response; regulation of phagocytosis; regulation of podosome assembly; regulation of transcription factor activity; respiratory burst after phagocytosis; transmembrane receptor protein tyrosine kinase signaling pathway
Research Articles on HCK
Similar Products
Product Notes
The HCK hck (Catalog #AAA2027347) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Hemopoietic Cell Kinase (HCK) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Hemopoietic Cell Kinase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the HCK hck for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hemopoietic Cell Kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
