Western Blot (WB)
(Anti-PYGO2 rabbit polyclonal antibody at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:41 kDa Observed band size:50 kDa)
Rabbit anti-Human PYGO2/Pygopus-2 Polyclonal Antibody | anti-PYGO2 antibody
Anti-PYGO2/Pygopus-2 Antibody, Rabbit Polyclonal
Gene Names
PYGO2; 1190004M21Rik
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
PYGO2/Pygopus-2; Polyclonal Antibody; Anti-PYGO2/Pygopus-2 Antibody; Rabbit Polyclonal; Pygopus-2/PYGO2 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-1190004M21Rik Antibody; Anti-PP7910 Antibody; anti-PYGO2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Pygopus-2/PYGO2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PYGO2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
ICC/IF: 1:500-1:2500
IP: 1-2uL/mg of lysate
ICC/IF: 1:500-1:2500
IP: 1-2uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the center region of the Human Pygopus-2/PYGO2
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Pygopus-2/PYGO2, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PYGO2 rabbit polyclonal antibody at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:41 kDa Observed band size:50 kDa)
Western Blot (WB)
(Anti-PYGO2 rabbit polyclonal antibody at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:41 kDa Observed band size:50 kDa)
Immunofluorescence (IF)
(Immunofluorescence staining of PYGO2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PYGO2 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of PYGO2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PYGO2 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.)
Immunoprecipitation (IP)
(PYGO2 was immunoprecipitated using: Lane A:0.5 mg MCF7 Whole Cell Lysate 1 uL anti-PYGO2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PYGO2 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 41 kDa Observed band size :54 kDa)
Immunoprecipitation (IP)
(PYGO2 was immunoprecipitated using: Lane A:0.5 mg MCF7 Whole Cell Lysate 1 uL anti-PYGO2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PYGO2 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 41 kDa Observed band size :54 kDa)
Related Product Information for anti-PYGO2 antibody
PYGO2 overexpression enhances primary tumor growth and local invasion to draining lymph nodes. Conversely, PYGO2 depletion inhibits prostate cancer cell invasion in vitro and progression of primary tumor and metastasis in vivo In clinical samples, PYGO2 upregulation is associated with higher Gleason score and metastasis to lymph nodes and bone. Silencing PYGO2 expression in patient-derived xenograft models impairs tumor progression. Finally, PYGO2 is necessary to enhance the transcriptional activation in response to ligand-induced Wnt/beta-catenin signaling.YGO2 functions as a driver oncogene in the 1q21.3 amplicon and may serve as a potential prognostic biomarker and therapeutic target for metastatic prostate cancer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,244 Da
NCBI Official Full Name
Homo sapiens pygopus family PHD finger 2 (PYGO2), mRNA
NCBI Official Synonym Full Names
pygopus family PHD finger 2
NCBI Official Symbol
PYGO2
NCBI Official Synonym Symbols
1190004M21Rik
NCBI Protein Information
pygopus homolog 2; pygopus 2
UniProt Protein Name
Pygopus homolog 2
Protein Family
UniProt Gene Name
PYGO2
UniProt Entry Name
PYGO2_HUMAN
Research Articles on PYGO2
Similar Products
Product Notes
The PYGO2 pygo2 (Catalog #AAA8101546) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PYGO2/Pygopus-2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PYGO2/Pygopus-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:1000 ICC/IF: 1:500-1:2500 IP: 1-2uL/mg of lysate. Researchers should empirically determine the suitability of the PYGO2 pygo2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PYGO2/Pygopus-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
