Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using phosphor-KDR (Tyr1214) antibody.)
Rabbit anti-Human, Mouse KDR Polyclonal Antibody | anti-phospho-KDR antibody
Anti-phospho-KDR (Tyr1214)
Reactivity
Human, Mouse
Applications
Immunohistochemistry
Purity
Affinity chromatography
Synonyms
KDR; Polyclonal Antibody; Anti-phospho-KDR (Tyr1214); anti-phospho-KDR antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the KDR protein phosphorylated at tyrosine 1214
Purity/Purification
Affinity chromatography
Form/Format
PBS (pH7.4) 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-phospho-KDR antibody
Immunohistochemistry (IHC)
Application Notes
IHC: 1:50-1:100
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1214
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using phosphor-KDR (Tyr1214) antibody.)
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using phosphor-KDR (Tyr1214) antibody.)
Related Product Information for anti-phospho-KDR antibody
Kinase insert domain receptor (KDR or VEGFR) is a growth factor receptor tyrosine kinase that plays a pivotal role in endothelial cell proliferation and differentiation. KDR binds VEGF with high affinity and this interaction is implicated in angiogenesis (1). The expression levels of VEGF and KDR are highly correlated during the normal development of the ocular vasculature in humans (1). Induction of angiogenesis is a critical step in tumor progression, and inhibitors of KDR have been demonstrated both to promote tumor regression and reduce metastatic potential in preclinical studies (2). Autophosphorylation of KDR occurs at Tyr951, Tyr996, Tyr1054 and Tyr1059 and phosphorylation at Tyr1214 is associated with interactions with downstream effector molecules (3).
Product Categories/Family for anti-phospho-KDR antibody
References
1. Neufeld, G. et al: Vascular endothelial growth factor (VEGF) and its receptors. FASEB J. 1999 Jan;13(1):9-22.
2. Zhu, Z. et al: Inhibition of tumor growth and metastasis by targeting tumor-associated angiogenesis with antagonists to the receptors of vascular endothelial growth factor. Invest New Drugs. 1999;17(3):195-212.
3. Lamalice L. et al: Phosphorylation of Tyr1214 within VEGFR-2 triggers the recruitment of Nck and activation of Fyn leading to SAPK2/p38 activation and endothelial cell migration in response to VEGF. J Biol Chem. 2006 Nov 10;281(45):34009-20.
2. Zhu, Z. et al: Inhibition of tumor growth and metastasis by targeting tumor-associated angiogenesis with antagonists to the receptors of vascular endothelial growth factor. Invest New Drugs. 1999;17(3):195-212.
3. Lamalice L. et al: Phosphorylation of Tyr1214 within VEGFR-2 triggers the recruitment of Nck and activation of Fyn leading to SAPK2/p38 activation and endothelial cell migration in response to VEGF. J Biol Chem. 2006 Nov 10;281(45):34009-20.
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Product Notes
The phospho-KDR (Catalog #AAA516037) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-KDR (Tyr1214) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KDR can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). IHC: 1:50-1:100. Researchers should empirically determine the suitability of the phospho-KDR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KDR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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