Rabbit anti-Human IRAK2 / IRAK-2 Polyclonal Antibody | anti-IRAK2 antibody

Rabbit Polyclonal to Human IRAK2 / IRAK-2

Cat. Num. AAA247326

• Gene Names: IRAK2; IRAK-2
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: IRAK2 / IRAK-2; Polyclonal Antibody; Rabbit Polyclonal to Human IRAK2 / IRAK-2; Anti-IRAK2 / IRAK-2 Antibody (C-Terminus) IHC-plus; IRAK2; Il1rak2; IRAK-2; Human IRAK2; anti-IRAK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: peptide corresponding to amino acids near the carboxy-terminus of human IRAK2
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Sequence Length: 625

• Applicable Applications for anti-IRAK2 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, ICC, IHC-P (5 ug/ml), WB (1 - 2 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human IRAK2.
• Immunogen Type: Synthetic peptide
• Immunogen: IRAK2 / IRAK-2 antibody was raised against synthetic peptide from human IRAK2.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-IRAK2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-IRAK2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-IRAK2 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of IRAK-2 in HeLa cells with IRAK-2 antibody at 10 ug/ml.)

Immunocytochemistry (ICC)

(Immunocytochemical staining of HeLa cells using IRAK2 antibody at 2 µg/ml.)

Western Blot (WB)

(Western blot of IRAK2 in HeLa (H) and K562 (K) whole cell lysate with IRAK2 antibody at 2 ug/ml.)

Related Product Information for anti-IRAK2 antibody

IRAK2, an IRAK type protein kinase, is a signal transducer for the immune response interleukin-1 receptor (IL-1R) signaling cascade. IRAK2 also has been associated with signaling cell death, potentially via the Fas-associated death domain protein/caspase-8 apoptotic pathway. Dominant negative forms of IRAK2 attenuate IL-1R-mediated NF-kappa B activation, suggesting that IRAK-2 may serve as a therapeutic target for inhibiting IL-1-induced inflammation.

Product Categories/Family for anti-IRAK2 antibody

Family: Protein Kinase; Subfamily: IRAK (Pelle)

NCBI and Uniprot Product Information

• NCBI GI #: 58530886
• NCBI GeneID: 3656
• NCBI Accession #: NP_001561.3
• NCBI GenBank Nucleotide #: NM_001570.3
• UniProt Accession #: O43187; Q08AG6; Q5K546; B4DQZ6
• Molecular Weight: 69,433 Da
• NCBI Official Full Name: interleukin-1 receptor-associated kinase-like 2
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 2
• NCBI Official Symbol: IRAK2
• NCBI Official Synonym Symbols: IRAK-2
• NCBI Protein Information: interleukin-1 receptor-associated kinase-like 2; interleukin-1 receptor associated kinase-2
• UniProt Protein Name: Interleukin-1 receptor-associated kinase-like 2
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: IRAK2
• UniProt Synonym Gene Names: IRAK-2
• UniProt Entry Name: IRAK2_HUMAN

NCBI Description

IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB. [provided by RefSeq, Jul 2008]

Uniprot Description

IRAK2: Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Interacts with MYD88. IL-1 stimulation leads to the formation of a signaling complex which dissociates from the IL-1 receptor following the binding of PELI1. Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

Protein type: Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; IRAK family

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: interleukin-1 receptor complex; cytoplasm; plasma membrane; endosome membrane; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein homodimerization activity; protein heterodimerization activity; ATP binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; activation of MAPK activity; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; inhibition of NF-kappaB transcription factor; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; JNK cascade; regulation of cytokine and chemokine mediated signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway