Rabbit anti-Human IPO11 Polyclonal Antibody | anti-IPO11 antibody

IPO11, CT (IPO11, RANBP11, Importin-11, Ran-binding protein 11) (PE)

Cat. Num. AAA6310717

• Gene Names: IPO11; RanBP11
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IPO11; Polyclonal Antibody; CT (IPO11; RANBP11; Importin-11; Ran-binding protein 11) (PE); Ran-binding protein 11; anti-IPO11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-IPO11 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: IPO11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 906~970 amino acids from the C-terminal region of human IPO11.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of IPO11 Antibody (C-term) in CEM cell line lysates (35ug/lane). IPO11 (arrow) was detected using the purified Pab.)

Related Product Information for anti-IPO11 antibody

IPO11 functions in nuclear protein import as nuclear transport receptor. It serves as receptor for nuclear localization signals (NLS) in cargo substrates and is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP-and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). It mediates the nuclear import of UBE2E3, and of RPL12 (By similarity).

Product Categories/Family for anti-IPO11 antibody

Antibodies; Binding Proteins

References

Plafker, S.M. et.al., Mol. Cell. Biol. 22 (4), 1266-1275 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 21707128
• NCBI GeneID: 51194
• UniProt Accession #: Q9UI26; Q8N5R2; Q9NSJ6; Q9NVB1; A6NGJ5; B4DZ73; D3DW98
• Molecular Weight: 112,535 Da
• NCBI Official Full Name: Importin 11
• NCBI Official Synonym Full Names: importin 11
• NCBI Official Symbol: IPO11
• NCBI Official Synonym Symbols: RanBP11
• NCBI Protein Information: importin-11; imp11; Ran binding protein 11; ran-binding protein 11
• UniProt Protein Name: Importin-11
• Protein Family: Importin
• UniProt Gene Name: IPO11
• UniProt Synonym Gene Names: RANBP11; Imp11; RanBP11
• UniProt Entry Name: IPO11_HUMAN

NCBI Description

Importins, including IPO11, are a members of the karyopherin/importin-beta family of transport receptors (see KPNB1; 602738) that mediate nucleocytoplasmic transport of protein and RNA cargoes (Plafker and Macara, 2000 [PubMed 11032817]).[supplied by OMIM, Sep 2008]

Uniprot Description

IPO11: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of UBE2E3, and of RPL12. Belongs to the importin beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Karyopherin; Nuclear import

Chromosomal Location of Human Ortholog: 5q12.1

Cellular Component: cytoplasm; nucleus

Molecular Function: Ran GTPase binding; protein transporter activity

Biological Process: ribosomal protein import into nucleus