Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Rabbit RPN1 Polyclonal Antibody | anti-RPN1 antibody
RPN1 Antibody
Gene Names
RPN1; OST1; RBPH1
Reactivity
Human, Mouse, Rat, MonkeyPredicted: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
RPN1; Polyclonal Antibody; RPN1 Antibody; D6Wsu137e; Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit; Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit precursor; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; EC 2.4.1.119; Oligosaccharyltransferase 1 homolog; oligosaccharyltransferase complex subunit (non-catalytic); OST1; RBPH1; Ribophorin I; Ribophorin-1; RPN-I; Rpn1; RPN1_HUMAN; anti-RPN1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Predicted: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Predicted: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
RPN1 Antibody detects endogenous levels of RPN1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
607
Applicable Applications for anti-RPN1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human RPN1.
Tissue Specificity
Expressed in all tissues tested.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Endoplasmic Reticulum
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Western Blot (WB)
(Western blot analysis of extracts from K-562 cells, using RPN1 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from K-562 cells, using RPN1 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from COS-7, using RPN1 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from COS-7, using RPN1 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-RPN1 antibody
Description: Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
Subunit Structure: Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).
Similarity: Belongs to the OST1 family.
Subunit Structure: Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).
Similarity: Belongs to the OST1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 69 kDa
Predicted: 69 kDa
Predicted: 69 kDa
NCBI Official Full Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
NCBI Official Synonym Full Names
ribophorin I
NCBI Official Symbol
RPN1
NCBI Official Synonym Symbols
OST1; RBPH1
NCBI Protein Information
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
UniProt Protein Name
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
Protein Family
UniProt Gene Name
RPN1
UniProt Synonym Gene Names
RPN-I
UniProt Entry Name
RPN1_HUMAN
NCBI Description
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq, Jul 2008]
Research Articles on RPN1
Similar Products
Product Notes
The RPN1 rpn1 (Catalog #AAA9611478) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPN1 Antibody reacts with Human, Mouse, Rat, Monkey Predicted: Pig, Bovine, Horse, Rabbit, Dog, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's RPN1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the RPN1 rpn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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