Rabbit anti-Human B2M  Monoclonal Antibody | anti-B2M  antibody

Rabbit anti Human B2M  Monoclonal Antibody

Cat. Num. AAA2544321

• Gene Names: B2M; IMD43
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity purification
• Synonyms: B2M ; Monoclonal Antibody; Rabbit anti Human B2M Monoclonal Antibody; B2M; CDABP0092; Hdcma22p; IMD43; anti-B2M antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 119

• Applicable Applications for anti-B2M antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Western Blot (WB) 1:20-1:50
• Immunogen: Recombinant Human B2M microglobulin protein
• Buffer: 0.2 um filtered solution in PBS with 5% trehalose
• Preparation and Storage: Store at -20°C.Avoid freeze / thaw cycles .

Western Blot (WB)

(Western Blot analysis of Hela cells using B2M Monoclonal Antibody at dilution of 1:20.)

Related Product Information for anti-B2M  antibody

B2M, also known as beta2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M (beta2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M (beta2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens.B2M (beta2- Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to thealpha3 chain. Unlikealpha3, beta2 has no transmembrane region. Directly above beta2 lies the alpha1 chain, which itself is lateral to the alpha2. In the absence of B2M (beta2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M (beta2 microglobulin) can indicate non-progression of HIV.

NCBI and Uniprot Product Information

• NCBI GI #: 823672134
• NCBI GeneID: 567
• NCBI Official Full Name: B2M, partial
• NCBI Official Synonym Full Names: beta-2-microglobulin
• NCBI Official Symbol: B2M
• NCBI Official Synonym Symbols: IMD43
• NCBI Protein Information: beta-2-microglobulin
• UniProt Protein Name: Beta-2-microglobulin
• UniProt Gene Name: B2M
• UniProt Entry Name: B2MG_HUMAN

NCBI Description

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014]

Uniprot Description

B2M: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Heterodimer of an alpha chain and a beta chain. Beta-2- microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. Belongs to the beta-2-microglobulin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: cytoplasm; early endosome membrane; endoplasmic reticulum lumen; extracellular region; extracellular space; focal adhesion; Golgi apparatus; Golgi membrane; membrane; phagocytic vesicle membrane; plasma membrane

Molecular Function: glycoprotein binding; identical protein binding; protein binding

Biological Process: antibacterial humoral response; antigen processing and presentation of endogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; innate immune response; iron ion homeostasis; positive regulation of protein binding; positive regulation of receptor-mediated endocytosis; positive regulation of T cell cytokine production; regulation of defense response to virus by virus; regulation of immune response; retinal homeostasis

Disease: Amyloidosis, Familial Visceral; Hypoproteinemia, Hypercatabolic