Rabbit anti-Human Internexin, alpha Polyclonal Antibody | anti-INA antibody

Internexin, alpha, ID (INA, 66kD Neurofilament Protein, Alpha-Internexin, Alpha Inx, FLJ18662, FLJ57501, MGC12702, NEF 5, NEF5, Neurofilament 5, Neurofilament 66, Neurofilament-66 NF 66, NF66, NF-66) (Biotin)

Cat. Num. AAA6483305

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Internexin; alpha; Polyclonal Antibody; ID (INA; 66kD Neurofilament Protein; Alpha-Internexin; Alpha Inx; FLJ18662; FLJ57501; MGC12702; NEF 5; NEF5; Neurofilament 5; Neurofilament 66; Neurofilament-66 NF 66; NF66; NF-66) (Biotin); anti-INA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human INA.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 499

• Applicable Applications for anti-INA antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human INA (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of INA (arrow) using rabbit polyclonal INA Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the INA gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with INA antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-INA antibody

Antibodies; Neurofilament

References

1. Armstrong, R.A., Eur. J. Neurol. 13(5), 528-532 (2006). 2. Suzuki, T., Eur. J. Neurosci. 21(2), 339-350 (2005). 3. Cairns, N.J., Am. J. Pathol. 164(6), 2153-2161 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 14249342
• NCBI Accession #: NP_116116
• NCBI GenBank Nucleotide #: NP_116116.1
• UniProt Accession #: Q16352
• Molecular Weight: 55.391
• NCBI Official Full Name: alpha-internexin
• UniProt Protein Name: Alpha-internexin
• Protein Family: Immune inhibitor
• UniProt Gene Name: INA
• UniProt Synonym Gene Names: NEF5; Alpha-Inx; NF-66
• UniProt Entry Name: AINX_HUMAN

Uniprot Description

INA: Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 10q24.33

Cellular Component: nucleoplasm; extracellular space; intermediate filament cytoskeleton; nuclear membrane; neurofilament

Molecular Function: structural constituent of cytoskeleton

Biological Process: substantia nigra development; neurofilament cytoskeleton organization and biogenesis; cell differentiation