Western Blot (WB)
(WB: Untreated (lane 1) and treated with sodium butyrate (lane 2) HeLa cell acid-extract was probed with MBS622059 at 0.5ug/ml.)
Rabbit Histone H3 Polyclonal Antibody | anti-HIST1H3A antibody
Histone H3, acetylated (Lys9) (MaxLight 550)
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Dot Blot, Flow Cytometry, Functional Assay, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Histone H3; Polyclonal Antibody; acetylated (Lys9) (MaxLight 550); anti-HIST1H3A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Histone H3 (Mr 17kD) acetylated at position Lys 9. Species Crossreactivity: mouse and rat
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Applicable Applications for anti-HIST1H3A antibody
Western Blot (WB), Dot Blot (DB), Flow Cytometry (FC/FACS), Chromatin Immunoprecipitation (ChIP)
Application Notes
WB: 1:10,000. Detects acetylated histone H3 on 10ug of sodium butyrate treated HeLa cell lysates.
Dot Blot: 1:1000
FC/FACS: Detected Histone H3, acetylated (Lys9) in 1x10e6 HEK293 cells
Applications are based on unconjugated antibody.
Dot Blot: 1:1000
FC/FACS: Detected Histone H3, acetylated (Lys9) in 1x10e6 HEK293 cells
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to aa1-12, ARTKQTAR(AcK)STG-C, where (AcK) denotes acetyl-lysine of human Histone H3, conjugated to KLH (NP_003520). Peptide sequence is identical in a wide range of animal and plant species.
Conjugate
MaxLight550
Positive Control
HeLa Cell Lysates
Antigen Modification
Acetylated
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(WB: Untreated (lane 1) and treated with sodium butyrate (lane 2) HeLa cell acid-extract was probed with MBS622059 at 0.5ug/ml.)
Western Blot (WB)
(WB: Untreated (lane 1) and treated with sodium butyrate (lane 2) HeLa cell acid-extract was probed with MBS622059 at 0.5ug/ml.)
Testing Data
(HEK293 lysate was resolved by electrophoresis, transferred to membrane and probed with MBS622059 (1:500). Proteins were visualized using an IgG (HRP) donkey anti-rabbit secondary antibody and a chemiluminescence detection system. Arrow indicates MBS622059 (~17kD).)
Testing Data
(HEK293 lysate was resolved by electrophoresis, transferred to membrane and probed with MBS622059 (1:500). Proteins were visualized using an IgG (HRP) donkey anti-rabbit secondary antibody and a chemiluminescence detection system. Arrow indicates MBS622059 (~17kD).)
Western Blot (WB)
(Western Blot Analysis: A431 (Lane1), C2C12 lysate (Lane2), HEK293 lysate (Lane 3), Hela lysate (Lane 4), HepG2 lysate (Lane 5), Jurkat lysate (Lane 6), L6 lysate (Lane 7), NIH/3T3 lysate (Lane 8), were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS622059 (1:500 dilution).)
Western Blot (WB)
(Western Blot Analysis: A431 (Lane1), C2C12 lysate (Lane2), HEK293 lysate (Lane 3), Hela lysate (Lane 4), HepG2 lysate (Lane 5), Jurkat lysate (Lane 6), L6 lysate (Lane 7), NIH/3T3 lysate (Lane 8), were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS622059 (1:500 dilution).)
Product Categories/Family for anti-HIST1H3A antibody
References
1. Su, R.C., et al., Nat. Genet. 36: 502-506 (2004). 2. Li, J., et al., J. Biol. Chem. 278: 1183-1191 (2003). 3. Bjerling, P., et al., Mol. Cell Biol. 22: 2170-2181 (2002). 4. Kruhlak, M., et al., J. Biol. Chem. 276: 38, 307-38, 319 (2001). 5. Nowak, S. & Corces, V., Genes Dev. 14: 3003-3013 (2000). 6. Salvador, L.M., et al., J. Biol. Chem. 276: 40, 146-40, 155 (2001). 7. Zhong, S., et al., J. Biol. Chem. 276: 33, 213-33, 219 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,404 Da
NCBI Official Full Name
histone H3.1
NCBI Official Synonym Full Names
histone cluster 1, H3a
NCBI Official Symbol
HIST1H3A
NCBI Official Synonym Symbols
H3/A; H3FA
NCBI Protein Information
histone H3.1; H3 histone family, member A; histone 1, H3a; histone H3/a
UniProt Protein Name
Histone H3.1t
Protein Family
UniProt Gene Name
HIST3H3
UniProt Synonym Gene Names
H3FT; H3/t; H3t
UniProt Entry Name
H31T_HUMAN
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Product Notes
The HIST1H3A hist3h3 (Catalog #AAA6480718) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3, acetylated (Lys9) (MaxLight 550) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Dot Blot (DB), Flow Cytometry (FC/FACS), Chromatin Immunoprecipitation (ChIP). WB: 1:10,000. Detects acetylated histone H3 on 10ug of sodium butyrate treated HeLa cell lysates. Dot Blot: 1:1000 FC/FACS: Detected Histone H3, acetylated (Lys9) in 1x10e6 HEK293 cells Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HIST1H3A hist3h3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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