Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Interleukin 4 Polyclonal Antibody | anti-IL4 antibody
Polyclonal Antibody to Interleukin 4 (IL4)
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded testis tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded testis tissue))
NCBI and Uniprot Product Information
Uniprot Description
IL4: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the IL-4/IL-13 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell cycle regulation; Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11 B1.3|11 31.97 cM
Cellular Component: external side of plasma membrane; extracellular region; extracellular space
Molecular Function: cytokine activity; cytokine receptor binding; growth factor activity; interleukin-4 receptor binding
Biological Process: B cell activation; B cell costimulation; cholesterol metabolic process; defense response to protozoan; immune response; innate immune response in mucosa; microglial cell activation; myeloid dendritic cell differentiation; negative regulation of acute inflammatory response; negative regulation of chronic inflammatory response; negative regulation of macrophage activation; negative regulation of nitric oxide biosynthetic process; negative regulation of osteoclast differentiation; negative regulation of T cell activation; negative regulation of transcription, DNA-dependent; positive regulation of activated T cell proliferation; positive regulation of B cell activation; positive regulation of B cell proliferation; positive regulation of cell proliferation; positive regulation of chemokine biosynthetic process; positive regulation of defense response to virus by host; positive regulation of immunoglobulin production; positive regulation of interleukin-10 production; positive regulation of interleukin-13 production; positive regulation of isotype switching to IgE isotypes; positive regulation of isotype switching to IgG isotypes; positive regulation of mast cell degranulation; positive regulation of MHC class II biosynthetic process; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein phosphorylation; positive regulation of T cell differentiation; positive regulation of T cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of tyrosine phosphorylation of STAT protein; regulation of immune response; regulation of inflammatory response; regulation of proton transport; T-helper 1 cell lineage commitment; T-helper 2 cell differentiation
Research Articles on IL4
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Product Notes
The IL4 il4 (Catalog #AAA2033891) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Interleukin 4 (IL4) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the IL4 il4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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