Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MKP-1/2 (Phospho-Ser296/318) antibody.)
Rabbit MKP1/2 Polyclonal Antibody | anti-MKP1/2 antibody
MKP1/2 antibody (Ser296/318)
Gene Names
MKP1; ARABIDOPSIS MITOGEN-ACTIVATED PROTEIN KINASE PHOSPHATASE 1; ATMKP1; mitogen-activated protein kinase phosphatase 1; MKP1
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
MKP1/2; Polyclonal Antibody; MKP1/2 antibody (Ser296/318); Polyclonal MKP1/2; Anti-MKP1/2; MKP 1/2 Phospho; dual specificity phosphatase 1; MKP 1/2; HVH1; Phospho MKP 1/2; phospho MKP 1/2; MKP1; MKP-1; PTPN10; CL100; DUSP1; anti-MKP1/2 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
MKP1/2 antibody detects endogenous levels of MKP1/2 only when phosphorylated at serine 296/318
Form/Format
Supplied in PBS buffer (without Mg2+and Ca2+), pH 7.4, 150mM NaCl with 0.02% sodium azide and 50% glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
784
Applicable Applications for anti-MKP1/2 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:5000
IHC: 1:50-1:100
ELISA: 1:5000
Biological Significance
MKP1/2 is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus.
Cross-Reactivity
Mouse, Rat
Immunogen
MKP1/2 antibody was raised in Rabbit using synthesized phosphopeptide derived from human MKP1/2 around the phosphorylation site of serine 296/318 (I-I-SP-P-N) as the immunogen
Preparation and Storage
Aliquot and store at -20 degree C. Avoid repeated freeze thaw cycles
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MKP-1/2 (Phospho-Ser296/318) antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MKP-1/2 (Phospho-Ser296/318) antibody.)
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, treated with heat shock, using MKP-1/2 (Phospho-Ser296/318) antibody.)
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, treated with heat shock, using MKP-1/2 (Phospho-Ser296/318) antibody.)
Related Product Information for anti-MKP1/2 antibody
Rabbit polyclonal MKP1/2 antibody (Ser296/318)
Product Categories/Family for anti-MKP1/2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85,964 Da
NCBI Official Full Name
mitogen-activated protein kinase phosphatase 1
NCBI Official Symbol
MKP1
NCBI Official Synonym Symbols
ARABIDOPSIS MITOGEN-ACTIVATED PROTEIN KINASE PHOSPHATASE 1; ATMKP1; mitogen-activated protein kinase phosphatase 1; MKP1
NCBI Protein Information
mitogen-activated protein kinase phosphatase 1
UniProt Protein Name
Protein-tyrosine-phosphatase MKP1
UniProt Gene Name
MKP1
UniProt Synonym Gene Names
AtMKP1
UniProt Entry Name
MKP1_ARATH
NCBI Description
MAP kinase phosphatase (MKP1)
Uniprot Description
Protein-tyrosine-phosphatase that acts as a negative regulator of MPK6 and MPK3 signaling by dephosphorylating and repressing MPK6 and MPK3. Modulates defense response by repressing salicylic acid (SA) production, camalexin biosynthesis and SNC1-mediated responses. Acts as a negative regulator of MPK6-mediated pathogen-associated molecular pattern (PAMP) responses, including MPK6 and MPK3 activation, accumulation of extracellular reactive oxygen species and inhibition of seedling growth. Involved in UV-B stress tolerance. May be involved in salt and genotoxic stress responses.
Research Articles on MKP1/2
Similar Products
Product Notes
The MKP1/2 mkp1 (Catalog #AAA839922) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MKP1/2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:1000 IHC: 1:50-1:100 ELISA: 1:5000. Researchers should empirically determine the suitability of the MKP1/2 mkp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MKP1/2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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