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Western Blot (WB) (Western Blot:Sample: Recombinant IL8R Beta, Mouse.)

Rabbit Interleukin 18 Receptor Accessory Protein (IL18RAP) Polyclonal Antibody | anti-IL18RAP antibody

Polyclonal Antibody to Interleukin 18 Receptor Accessory Protein (IL18RAP)

Gene Names
Il18rap; AcPL; IL-18RAcP; IL-1RAcPL; IL-18Rbeta; IL-18R-beta
Reactivity
Mus musculus (Mouse)
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Interleukin 18 Receptor Accessory Protein (IL18RAP); Polyclonal Antibody; Polyclonal Antibody to Interleukin 18 Receptor Accessory Protein (IL18RAP); anti-IL18RAP antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mus musculus (Mouse)
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-IL18RAP antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-3ug/mL
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Cross Reactivity
Rat
Traits
Liquid
Immunogen
Recombinant IL18R Beta (Asn396~Ser548 (Accession # Q9Z2B1)) expressed in E.coli ( MBS2009868 ).
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot:Sample: Recombinant IL8R Beta, Mouse.)

Western Blot (WB) (Western Blot:Sample: Recombinant IL8R Beta, Mouse.)

Western Blot (WB)

(Western Blot:Sample: Mouse Serum;Primary Ab: 2ug/ml Rabbit Anti-Mouse IL18RAP AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Western Blot (WB) (Western Blot:Sample: Mouse Serum;Primary Ab: 2ug/ml Rabbit Anti-Mouse IL18RAP AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Mouse Stomach Tissue;Primary Ab: 30ug/ml Rabbit Anti-Mouse IL18RAP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ))

Immunohistochemistry (IHC) (DAB staining on IHC-P;Samples: Mouse Stomach Tissue;Primary Ab: 30ug/ml Rabbit Anti-Mouse IL18RAP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ))

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded liver tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded liver tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Mouse Uterus Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P;Samples: Mouse Uterus Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
interleukin-18 receptor accessory protein
NCBI Official Synonym Full Names
interleukin 18 receptor accessory protein
NCBI Official Symbol
Il18rap
NCBI Official Synonym Symbols
AcPL; IL-18RAcP; IL-1RAcPL; IL-18Rbeta; IL-18R-beta
NCBI Protein Information
interleukin-18 receptor accessory protein
UniProt Protein Name
Interleukin-18 receptor accessory protein
UniProt Gene Name
Il18rap
UniProt Synonym Gene Names
IL-18 receptor accessory protein; IL-18RAcP; AcPL; IL-1RAcPL; IL-18R-beta; IL-18Rbeta

NCBI Description

Interleukin-18 (or interferon-gamma inducing factor) is a proinflammatory cytokine that induces cell-mediated immunity following microbial infection. This gene encodes a member of the interleukin-1 receptor family. The encoded protein is an accessory subunit of the receptor for interleukin-18 and mediates signaling through this cytokine. Mice lacking this gene exhibit a defective cell-mediated immune response. [provided by RefSeq, Jan 2014]

Uniprot Description

IL18RAP: Required for the high affinity binding of interleukin 18 (IL-18) to its receptor complex. Together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK. Belongs to the interleukin-1 receptor family.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1|1 B

Cellular Component: integral component of membrane; membrane

Molecular Function: interleukin-1 receptor activity

Biological Process: cell proliferation; interferon-gamma production; interleukin-6 production; neutrophil activation; positive regulation of natural killer cell mediated cytotoxicity; signal transduction

Research Articles on IL18RAP

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Product Notes

The IL18RAP il18rap (Catalog #AAA2006814) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Interleukin 18 Receptor Accessory Protein (IL18RAP) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 18 Receptor Accessory Protein (IL18RAP) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-3ug/mL Immunohistochemistry: 5-30ug/mL Immunocytochemistry: 5-30ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the IL18RAP il18rap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 18 Receptor Accessory Protein (IL18RAP), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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