Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Interleukin 18 Receptor Accessory Protein (IL18RAP) Polyclonal Antibody | anti-IL18RAP antibody
Polyclonal Antibody to Interleukin 18 Receptor Accessory Protein (IL18RAP)
Gene Names
Il18rap; AcPL; IL-18RAcP; IL-1RAcPL; IL-18Rbeta; IL-18R-beta
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Interleukin 18 Receptor Accessory Protein (IL18RAP); Polyclonal Antibody; Polyclonal Antibody to Interleukin 18 Receptor Accessory Protein (IL18RAP); anti-IL18RAP antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against IL18RAP. It has been selected for its ability to recognize IL18RAP in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- QTNS IWSYICRPRI RSPQDMACCI KTVLEVKPQR NVSCGNTAQD EQVLLLGSTG SIHCPSLSCQ SDVQSPEMTW YKDGRLLPEH KKNPIEMADI YVFNQGLYVC DYTQSDNVSS WTVRAVVKVR
MGHHHHHHSGSEF- QTNS IWSYICRPRI RSPQDMACCI KTVLEVKPQR NVSCGNTAQD EQVLLLGSTG SIHCPSLSCQ SDVQSPEMTW YKDGRLLPEH KKNPIEMADI YVFNQGLYVC DYTQSDNVSS WTVRAVVKVR
Sequence Length
599
Applicable Applications for anti-IL18RAP antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant IL18RAP (Gln117~Arg240) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2057580
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
70,094 Da
NCBI Official Full Name
interleukin-18 receptor accessory protein
NCBI Official Synonym Full Names
interleukin 18 receptor accessory protein
NCBI Official Symbol
Il18rap
NCBI Official Synonym Symbols
AcPL; IL-18RAcP; IL-1RAcPL; IL-18Rbeta; IL-18R-beta
NCBI Protein Information
interleukin-18 receptor accessory protein
UniProt Protein Name
Interleukin-18 receptor accessory protein
Protein Family
UniProt Gene Name
Il18rap
UniProt Synonym Gene Names
IL-18 receptor accessory protein; IL-18RAcP; AcPL; IL-1RAcPL; IL-18R-beta; IL-18Rbeta
NCBI Description
Interleukin-18 (or interferon-gamma inducing factor) is a proinflammatory cytokine that induces cell-mediated immunity following microbial infection. This gene encodes a member of the interleukin-1 receptor family. The encoded protein is an accessory subunit of the receptor for interleukin-18 and mediates signaling through this cytokine. Mice lacking this gene exhibit a defective cell-mediated immune response. [provided by RefSeq, Jan 2014]
Uniprot Description
IL18RAP: Required for the high affinity binding of interleukin 18 (IL-18) to its receptor complex. Together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK. Belongs to the interleukin-1 receptor family.
Protein type: Cell surface; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1|1 B
Cellular Component: integral component of membrane; membrane
Molecular Function: interleukin-1 receptor activity
Biological Process: cell proliferation; interferon-gamma production; interleukin-6 production; neutrophil activation; positive regulation of natural killer cell mediated cytotoxicity; signal transduction
Research Articles on IL18RAP
Similar Products
Product Notes
The IL18RAP il18rap (Catalog #AAA2007189) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Interleukin 18 Receptor Accessory Protein (IL18RAP) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 18 Receptor Accessory Protein (IL18RAP) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the IL18RAP il18rap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- QTNS IWSYICRPRI RSPQDMACCI KTVLEVKPQR NVSCGNTAQD EQVLLLGSTG SIHCPSLSCQ SDVQSPEMTW YKDGRLLPEH KKNPIEMADI YVFNQGLYVC DYTQSDNVSS WTVRAVVKVR. It is sometimes possible for the material contained within the vial of "Interleukin 18 Receptor Accessory Protein (IL18RAP), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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