Rabbit anti-Human IRF1 Polyclonal Antibody | anti-IRF1 antibody

IRF1, CT (IRF1, Interferon regulatory factor 1) (Azide free) (HRP)

Cat. Num. AAA6310887

• Gene Names: IRF1; MAR; IRF-1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IRF1; Polyclonal Antibody; CT (IRF1; Interferon regulatory factor 1) (Azide free) (HRP); Interferon regulatory factor 1; anti-IRF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-IRF1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: IRF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-293 amino acids from the C-terminal region of human IRF1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(IRF1 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the IRF1 antibody detected the IRF1 protein (arrow).)

Related Product Information for anti-IRF1 antibody

IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion.

Product Categories/Family for anti-IRF1 antibody

Antibodies; Interferon

References

Silva, L.K., et al. Eur. J. Hum. Genet. 18(11):1221-1227(2010). Matsuzaki, S., et al. J. Immunol. 185(8):4863-4872(2010). Antonios, D., et al. J. Immunol. 185(1):89-98(2010). Lou, Y.J., et al. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 27(3):255-258(2010). Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 196049386
• NCBI GeneID: 3659
• NCBI Accession #: NP_002189.1
• NCBI GenBank Nucleotide #: NM_002198
• UniProt Accession #: P10914; Q96GG7
• Molecular Weight: 36,502 Da
• NCBI Official Full Name: Homo sapiens interferon regulatory factor 1 (IRF1), mRNA
• NCBI Official Synonym Full Names: interferon regulatory factor 1
• NCBI Official Symbol: IRF1
• NCBI Official Synonym Symbols: MAR; IRF-1
• NCBI Protein Information: interferon regulatory factor 1; interferon regulatory factor 1 isoform +I9; interferon regulatory factor 1 isoform d78; interferon regulatory factor 1 isoform delta4; interferon regulatory factor 1 isoform delta7
• UniProt Protein Name: Interferon regulatory factor 1
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: IRF1
• UniProt Synonym Gene Names: IRF-1
• UniProt Entry Name: IRF1_HUMAN

NCBI Description

IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion. [provided by RefSeq, Jul 2008]

Uniprot Description

IRF1: a transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. These include the regulation of IFN and IFN-inducible genes, host response to viral and bacterial infections, regulation of many genes expressed during hematopoiesis, inflammation, immune responses and cell proliferation and differentiation, regulation of the cell cycle and induction of growth arrest and programmed cell death following DNA damage. Stimulates both innate and acquired immune responses through the activation of specific target genes and can act as a transcriptional activator and repressor regulating target genes by binding to an interferon- stimulated response element (ISRE) in their promoters. Its target genes for transcriptional activation activity include: genes involved in anti-viral response, such as IFN-alpha/beta, DDX58, TRAIL, OAS1/2, PIAS1, PKR and RSAD2; antibacterial response, such as iNOS; anti- proliferative response, such as p53, LOX and CDKN1A; apoptosis, such as PUMA, CASP1, CASP7 and CASP8; immune response, such as IL7, IL12A/B and IL15, COX-2 and CYBB; DNA damage responses and DNA repair, such as POLQ; MHC class I expression, such as TAP1, PSMB9, PSME1, PSME2 and B2M and MHC class II expression, such as CIITA. Represses genes involved in anti-proliferative response, such as survivin, CCNB1, CCNE1, CDK1, CDK2 and CDK4 and in immune response, such as FOXP3, IL4, ANXA2 and TLR4. Stimulates p53-dependent transcription through enhanced recruitment of EP300 leading to increased acetylation of p53. Plays an important role in immune response directly affecting NK maturation and activity, macrophage production of IL12, Th1 development and maturation of CD8+ T-cells. Also implicated in the differentiation and maturation of dendritic cells and in the suppression of regulatory T (Treg) cells development. Acts as a tumor suppressor and plays a role not only in antagonism of tumor cell growth but also in stimulating an immune response against tumor cells. Defects in IRF1 are a cause of gastric adenocarcinoma (GASC), accounting for most of all gastric malignant tumors. Deletions or rearrangements of IRF1 can occur in preleukemic myelodysplastic syndrome (MDS) and acute myelogenous leukemia (AML).

Protein type: Transcription factor; Tumor suppressor

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: nucleoplasm; cytoplasm; nuclear chromatin; cytosol; nucleus

Molecular Function: protein binding; DNA binding

Biological Process: transcription from RNA polymerase II promoter; CD8-positive, alpha-beta T cell differentiation; apoptosis; regulation of cell cycle; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; regulation of MyD88-dependent toll-like receptor signaling pathway; regulation of innate immune response; negative regulation of cell proliferation; regulation of adaptive immune response; positive regulation of interleukin-12 biosynthetic process; negative regulation of regulatory T cell differentiation; positive regulation of interferon-beta production; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; cell cycle arrest; blood coagulation; defense response to virus

Disease: Gastric Cancer; Lung Cancer