Rabbit anti-Rat Interleukin 1 Beta Polyclonal Antibody | anti-IL1B antibody

Interleukin 1 Beta (IL1 beta, Catabolin, H1, Hematopoietin 1, IFN beta Inducing Factor, IL 1, IL 1 beta, IL 1B, IL-1 beta, IL1 Beta, IL1B, IL1B_HUMAN, IL1F2, Interleukin 1 beta Precursor, Interleukin-1 beta, LAF, OAF, Osteoclast Activating Factor, Preinte

Cat. Num. AAA6460740

• Reactivity: Rat
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Interleukin 1 Beta; Polyclonal Antibody; Interleukin 1 Beta (IL1 beta; Catabolin; H1; Hematopoietin 1; IFN beta Inducing Factor; IL 1; IL 1 beta; IL 1B; IL-1 beta; IL1 Beta; IL1B; IL1B_HUMAN; IL1F2; Interleukin 1 beta Precursor; Interleukin-1 beta; LAF; OAF; Osteoclast Activating Factor; Preinte; anti-IL1B antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat IL1 beta.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.

• Applicable Applications for anti-IL1B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions.; ELISA: 0.1-0.5ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to rat IL1 beta expressed in E. coli. (Position: V117-S268). Species sequence homology: human (78%) and mouse (90%)
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months after receipt at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Anti-IL1 beta antibody, All lanes: Anti-IL1 beta at 0.5ug/ml WB: Rat Testis Tissue Lysate at 40ug Predicted band size: 31KD Observed band size: 35KD)

Product Categories/Family for anti-IL1B antibody

Antibodies; Interleukin

References

1. Takahashi, J. L.; Giuliani, F.; Power, C.; Imai, Y.; Yong, V. W.: Interleukin-1-beta promotes oligodendrocyte death through glutamate excitotoxicity. Ann. Neurol. 53: 588-595, 2003. 2. Baek, S. H.; Ohgi, K. A.; Rose, D. W.; Koo, E. H.; Glass, C. K.; Rosenfeld, M. G.: Exchange of N-CoR corepressor and Tip60 coactivator complexes links gene expression by NF-kappa-B and beta-amyloid precursor protein. Cell 110: 55-67, 2002. 3. Voronov, E.; Shouval, D. S.; Krelin, Y.; Cagnano, E.; Benharroch, D.; Iwakura, Y.; Dinarello, C. A.; Apte, R. N.: IL-1 is required for tumor invasiveness and angiogenesis. Proc. Nat. Acad. Sci. 100: 2645-2650, 2003. 4. Chen, C.-N.; Li, Y.-S. J.; Yeh, Y.-T.; Lee, P.-L.; Usami, S.; Chien, S.; Chiu, J.-J.: Synergistic roles of platelet-derived growth factor-BB and interleukin-1-beta in phenotypic modulation of human aortic smooth muscle cells. Proc. Nat. Acad. Sci. 103: 2665-2670, 2006.

NCBI and Uniprot Product Information

• NCBI GI #: 2497335
• NCBI GeneID: 24494
• UniProt Accession #: Q63264
• Molecular Weight: 30,644 Da
• NCBI Official Full Name: Interleukin-1 beta
• NCBI Official Synonym Full Names: interleukin 1 beta
• NCBI Official Symbol: Il1b
• NCBI Protein Information: interleukin-1 beta; IL-1 beta
• UniProt Protein Name: Interleukin-1 beta
• Protein Family: Interleukin
• UniProt Gene Name: Il1b
• UniProt Synonym Gene Names: IL-1 beta
• UniProt Entry Name: IL1B_RAT

NCBI Description

an inflammatory cytokine [RGD, Feb 2006]

Uniprot Description

IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family.

Protein type: Cytokine

Cellular Component: extracellular space; extracellular region; vesicle; secretory granule

Molecular Function: protein domain specific binding; interleukin-1 receptor binding; cytokine activity

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of JNK activity; negative regulation of MAP kinase activity; positive regulation of nitric oxide biosynthetic process; negative regulation of glutamate secretion; glycoprotein metabolic process; positive regulation of apoptosis; activation of MAPK activity; positive regulation of interleukin-2 biosynthetic process; positive regulation of transcription, DNA-dependent; germ cell programmed cell death; response to glucocorticoid stimulus; negative regulation of insulin receptor signaling pathway; positive regulation of NF-kappaB import into nucleus; positive regulation of glial cell differentiation; positive regulation of lipid catabolic process; response to lipopolysaccharide; fever; response to organic cyclic substance; positive regulation of membrane protein ectodomain proteolysis; response to carbohydrate stimulus; activation of NF-kappaB transcription factor; elevation of cytosolic calcium ion concentration; response to vitamin D; pentacyclic triterpenoid metabolic process; positive regulation of phagocytosis; positive regulation of T cell proliferation; positive regulation of astrocyte differentiation; response to drug; neutrophil chemotaxis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of heterotypic cell-cell adhesion; positive regulation of mitosis; interleukin-1 beta production; positive regulation of interleukin-6 production; social behavior; response to organic nitrogen; purine base metabolic process; negative regulation of neuron differentiation; positive regulation of angiogenesis; response to ethanol; response to heat; positive regulation of cell division; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of lipid metabolic process; leukocyte migration; response to peptide hormone stimulus; estrogen metabolic process; sequestering of triacylglycerol; wound healing; positive regulation of interleukin-6 biosynthetic process; response to morphine; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; response to L-ascorbic acid; chronic inflammatory response to antigenic stimulus; response to estradiol stimulus; positive regulation of stress-activated MAPK cascade; negative regulation of neurogenesis; positive regulation of interleukin-8 production; negative regulation of cell proliferation; learning and/or memory; negative regulation of lipid catabolic process; hyaluronan biosynthetic process; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; response to gamma radiation; inflammatory response; regulation of I-kappaB kinase/NF-kappaB cascade; response to nutrient; aging; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; positive regulation of immature T cell proliferation in the thymus; memory; response to ATP; ovulation; polyketide metabolic process; positive regulation of interferon-gamma production; positive regulation of chemokine biosynthetic process; response to ozone; positive regulation of prostaglandin secretion; response to hypoxia; positive regulation of fever; immune response; positive regulation of protein amino acid phosphorylation; regulation of insulin secretion