Rabbit OTUB1 Polyclonal Antibody | anti-OTUB1 antibody

OTUB1 polyclonal antibody

Cat. Num. AAA3014711

• Gene Names: OTUB1; OTB1; OTU1; HSPC263
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: OTUB1; Polyclonal Antibody; OTUB1 polyclonal antibody; Ubiquitin thioesterase OTUB1; Deubiquitinating enzyme OTUB1; OTU domain-containing ubiquitin aldehyde-binding protein 1; Otubain-1; hOTU1; Ubiquitin-specific-processing protease OTUB1; OTB1; OTU1; HSPC263; anti-OTUB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: OTUB1 polyclonal antibody detects endogenous levels of OTUB1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-OTUB1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic peptide, corresponding to Human OTUB1
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of OTUB1 polyclonal antibody at 1:500 dilutionLaneA: Hela whole cell lysateLaneB: HEK293T whole cell lysateLaneC: NIH3T3 whole cell lysateLaneD: The Brain tissue lysate of Mouse)

Related Product Information for anti-OTUB1 antibody

Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs). DUBs are categorized into 5 subfamilies: USP, UCH, OTU, MJD, and JAMM. The OTU subfamily comprises a group of approximately 100 putative cysteine proteases that are homologous to the ovarian tumor gene product of Drosophila. OTUB1 and OTUB2 (OTU domain-containing Ubal-binding proteins) display no significant similarity to any known DUB, but are close homologs and possess an OTU domain that contains conserved cysteine, histidine, and aspartate residues that define the putative catalytic triad of cysteine proteases. Furthermore, sequence analysis of OTUB1 and OTUB2 reveals the presence of putative Ub-interaction motifs (UIMs) and Ub-associated domains (UBAs), which are characteristic of proteins that regulate protein ubiquitination. OTUB1 and OTUB2 also possess a putative nuclear localization signal (NLS) and a consensus LxxLL motif, which mediates the interaction between transcriptional co-activators and nuclear hormone receptors.OTUB1 exists as two isoforms that are generated by alternative splicing; the shorter 31kDa isoform is ubiquitously expressed, while the longer 35kDa isoform (ARF-1) has a more restricted expression pattern and is mostly detected in lymphoid organs. Biochemical analysis has demonstrated that OTUB1 has a preference for cleaving K48-linked polyubiquitin chains over K63-linked polyubiquitin chains and is capable of cleaving NEDD8, but not SUMO-1, -2, and -3 or ISG15 conjugates. OTUB1 isoforms have been implicated in anergy induction in CD4+ T cells by regulating the stability of the E3 ligase GRAIL. More recently, OTUB1 was found to bind to and inhibit the E2 activity of UBE2N through a novel mechanism not involving OTUB1 DUB activity, thus compromising the ability of the E3 ligase RNF168 to drive ubiquitination-dependent repair of DNA double-strand lesions. OTUB1 also appears to suppress MDM2-dependent ubiquitination of p53 independent of its catalytic activity, primarily by suppressing the activity of the MDM2 cognate E2 UbcH5.

NCBI and Uniprot Product Information

• NCBI GI #: 109148508
• NCBI GeneID: 55611
• NCBI Accession #: NP_060140.2
• NCBI GenBank Nucleotide #: NM_017670.2
• UniProt Accession #: Q96FW1; Q32Q78; Q96II3; Q9NXQ4; Q9P0B8
• Molecular Weight: 35,285 Da
• NCBI Official Full Name: ubiquitin thioesterase OTUB1
• NCBI Official Synonym Full Names: OTU deubiquitinase, ubiquitin aldehyde binding 1
• NCBI Official Symbol: OTUB1
• NCBI Official Synonym Symbols: OTB1; OTU1; HSPC263
• NCBI Protein Information: ubiquitin thioesterase OTUB1; OTU domain, ubiquitin aldehyde binding 1; OTU domain-containing ubiquitin aldehyde-binding protein 1; OTU-domain Ubal-binding 1; deubiquitinating enzyme OTUB1; otubain-1; ubiquitin-specific protease otubain 1; ubiquitin-speci
• UniProt Protein Name: Ubiquitin thioesterase OTUB1
• Protein Family: Ubiquitin thioesterase
• UniProt Gene Name: OTUB1
• UniProt Synonym Gene Names: OTB1; OTU1; hOTU1
• UniProt Entry Name: OTUB1_HUMAN

NCBI Description

The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

OTUB1: a protease that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occur when T-cell are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Two alternatively spliced isoforms are known.

Protein type: Ubiquitin-specific protease; EC 3.4.19.12; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: cytoplasm

Molecular Function: protein binding; ubiquitin binding; ubiquitin protein ligase binding; ubiquitin-specific protease activity; NEDD8-specific protease activity

Biological Process: immune system process; proteolysis; DNA repair; response to DNA damage stimulus