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Western Blot (WB)
(Western Blot: Sample: Human MCF7 cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse IRF6 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Interferon Regulatory Factor 6 Polyclonal Antibody | anti-IRF6 antibody
Polyclonal Antibody to Interferon Regulatory Factor 6 (IRF6)
Gene Names
Irf6; mirf6; AI876454; E230028I05Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Interferon Regulatory Factor 6; Polyclonal Antibody; Polyclonal Antibody to Interferon Regulatory Factor 6 (IRF6); anti-IRF6 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against IRF6. It has beenselected for its ability to recognize IRF6 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- L EQSQHHVPIQ DTFPFLNING SPMAPASVGN CSVGNCSPES VWPKTEPLEM EVPQAPIQPF YSSPELWISS LPMTDLDIKF QYRGKEYGQT MTVSNPQGCR LFYGDLGPMP DQEELFGPVS LEQVKFPGPE HITNEKQKLF TSKLLDVMDR GLILEVSGHA IYAIRLCQCK VYWSGPCAPS LAAPNLIERQ KKVKLFCLET FLSELIAHQK GQIEKQPPFE IYLCFGEEWP DGKPLERKLI LVQVIPVVAR MIYEMFSGDF TRSFDSGSVR LQISTPDIKD NIVAQLKQLY RILQT
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- L EQSQHHVPIQ DTFPFLNING SPMAPASVGN CSVGNCSPES VWPKTEPLEM EVPQAPIQPF YSSPELWISS LPMTDLDIKF QYRGKEYGQT MTVSNPQGCR LFYGDLGPMP DQEELFGPVS LEQVKFPGPE HITNEKQKLF TSKLLDVMDR GLILEVSGHA IYAIRLCQCK VYWSGPCAPS LAAPNLIERQ KKVKLFCLET FLSELIAHQK GQIEKQPPFE IYLCFGEEWP DGKPLERKLI LVQVIPVVAR MIYEMFSGDF TRSFDSGSVR LQISTPDIKD NIVAQLKQLY RILQT
Sequence Length
372
Applicable Applications for anti-IRF6 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
IRF6 (Leu150~Thr445)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Human, Mouse
Conjugate
No Conjugate
Immunogen
Recombinant IRF6 (Leu150~Thr445) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2061047
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Human MCF7 cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse IRF6 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human MCF7 cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse IRF6 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
Immunohistochemistry (IHC)
(FITC staining on IHC-P;Simple: A549 cells))
Immunohistochemistry (IHC)
(FITC staining on IHC-P;Simple: A549 cells))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,111 Da
NCBI Official Full Name
interferon regulatory factor 6
NCBI Official Synonym Full Names
interferon regulatory factor 6
NCBI Official Symbol
Irf6
NCBI Official Synonym Symbols
mirf6; AI876454; E230028I05Rik
NCBI Protein Information
interferon regulatory factor 6
UniProt Protein Name
Interferon regulatory factor 6
Protein Family
UniProt Gene Name
Irf6
UniProt Synonym Gene Names
IRF-6
Uniprot Description
IRF6: Probable DNA-binding transcriptional activator. Key determinant of the keratinocyte proliferation-differentiation switch involved in appropriate epidermal development. Plays a role in regulating mammary epithelial cell proliferation. May regulate WDR65 transcription. Interacts with SERPINB5. Expressed in normal mammary epithelial cells. Expression is reduced or absent in breast carcinomas. Belongs to the IRF family.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 1 H6|1 97.6 cM
Cellular Component: cytoplasm; cytosol; nucleus
Molecular Function: DNA binding; DNA binding transcription factor activity; sequence-specific DNA binding
Biological Process: cell cycle arrest; cell development; cell differentiation; keratinocyte differentiation; keratinocyte proliferation; negative regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of transcription, DNA-templated; skin development; transcription, DNA-dependent
Research Articles on IRF6
Similar Products
Product Notes
The IRF6 irf6 (Catalog #AAA2028382) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Interferon Regulatory Factor 6 (IRF6) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Interferon Regulatory Factor 6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the IRF6 irf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- L EQSQHHVPIQ DTFPFLNING SPMAPASVGN CSVGNCSPES VWPKTEPLEM EVPQAPIQPF YSSPELWISS LPMTDLDIKF QYRGKEYGQT MTVSNPQGCR LFYGDLGPMP DQEELFGPVS LEQVKFPGPE HITNEKQKLF TSKLLDVMDR GLILEVSGHA IYAIRLCQCK VYWSGPCAPS LAAPNLIERQ KKVKLFCLET FLSELIAHQK GQIEKQPPFE IYLCFGEEWP DGKPLERKLI LVQVIPVVAR MIYEMFSGDF TRSFDSGSVR LQISTPDIKD NIVAQLKQLY RILQT. It is sometimes possible for the material contained within the vial of "Interferon Regulatory Factor 6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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