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Western Blot (WB) (Western Blot: Sample: Human Hela cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse GATA2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Mouse GATA Binding Protein 2 Polyclonal Antibody | anti-GATA2 antibody

Polyclonal Antibody to GATA Binding Protein 2 (GATA2)

Gene Names
Gata2; Gata-2
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
GATA Binding Protein 2; Polyclonal Antibody; Polyclonal Antibody to GATA Binding Protein 2 (GATA2); anti-GATA2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GATA2. It has beenselected for its ability to recognize GATA2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-TVSP FSKTPLHPSA AGAPGGPLSV YPGAAGGSGG GSGSSVASLT PTAAHSGSHL FGFPPTPPKE VSPDPSTTGA ASPASSSAGG SVARGEDKDG VKYQVSLSES MKMEGGSPLR PGLATMGTQP ATHHPIPTYP SYVPAAAHDY GSSLFHPGGF LGGPASSFTP KQRSKARSCS EGRECVNCGA TATPLWRRDG TGHYLCNACG LYHKMNGQNR PLIKPKRR
Applicable Applications for anti-GATA2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
GATA2 (Thr117~Arg338)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Human, Mouse
Conjugate
No Conjugate
Immunogen
Recombinant GATA2 (Thr117~Arg338) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2041168
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Human Hela cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse GATA2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Human Hela cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse GATA2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded Kidney tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,468 Da
NCBI Official Full Name
endothelial transcription factor GATA-2
NCBI Official Synonym Full Names
GATA binding protein 2
NCBI Official Symbol
Gata2
NCBI Official Synonym Symbols
Gata-2
NCBI Protein Information
endothelial transcription factor GATA-2
UniProt Protein Name
Endothelial transcription factor GATA-2
Protein Family
UniProt Gene Name
Gata2

Uniprot Description

GATA2: Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'. Endothelial cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 6 D1|6 39.2 cM

Cellular Component: nucleoplasm; nucleus; protein complex; transcription factor complex

Molecular Function: chromatin binding; DNA binding; DNA binding transcription factor activity; metal ion binding; protein binding; sequence-specific DNA binding; transcription factor binding; zinc ion binding

Biological Process: cell differentiation; cell differentiation in hindbrain; cell fate determination; cell maturation; central nervous system neuron development; commitment of a neuronal cell to a specific type of neuron in the forebrain; elevation of cytosolic calcium ion concentration; embryonic placenta development; hemopoiesis; homeostasis of number of cells within a tissue; inner ear morphogenesis; negative regulation of fat cell differentiation; negative regulation of fat cell proliferation; negative regulation of macrophage differentiation; negative regulation of myeloid cell differentiation; negative regulation of neural precursor cell proliferation; negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter; neuron differentiation; neuron fate commitment; neuron migration; phagocytosis; pituitary gland development; positive regulation of angiogenesis; positive regulation of erythrocyte differentiation; positive regulation of mast cell degranulation; positive regulation of megakaryocyte differentiation; positive regulation of neuron differentiation; positive regulation of phagocytosis; positive regulation of phagocytosis, engulfment; positive regulation of transcription from RNA polymerase II promoter; regulation of forebrain neuron differentiation; regulation of histone acetylation; regulation of primitive erythrocyte differentiation; regulation of transcription, DNA-templated; somatic stem cell maintenance; transcription, DNA-dependent; urogenital system development; ventral spinal cord interneuron differentiation

Research Articles on GATA2

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Product Notes

The GATA2 gata2 (Catalog #AAA2027538) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to GATA Binding Protein 2 (GATA2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GATA Binding Protein 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the GATA2 gata2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-TVS P FSKTPLHPSA AGAPGGPLSV YPGAAGGSGG GSGSSVASLT PTAAHSGSHL FGFPPTPPKE VSPDPSTTGA ASPASSSAGG SVARGEDKDG VKYQVSLSES MKMEGGSPLR PGLATMGTQP ATHHPIPTYP SYVPAAAHDY GSSLFHPGGF LGGPASSFTP KQRSKARSCS EGRECVNCGA TATPLWRRDG TGHYLCNACG LYHKMNGQNR PLIKPKRR. It is sometimes possible for the material contained within the vial of "GATA Binding Protein 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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