Rabbit Integrin, beta5 Polyclonal Antibody | anti-Itgb5 antibody

Integrin, beta5

Cat. Num. AAA618504

• Gene Names: Itgb5; [b]5; [b]-5; [b]5A; [b]5B; beta5; ESTM23; beta-5; AA475909; AI874634
• Reactivity: Hamster, Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, immunoassay, Radioimmunoassay
• Purity: Serum; Whole rabbit serum.
• Synonyms: Integrin; beta5; Polyclonal Antibody; Anti -Integrin; anti-Itgb5 antibody

• Host: Rabbit
• Reactivity: Hamster, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Integrin beta 5. Specificity determined by immunoprecipitation from detergent extract of 35S methionine labeled cells. No crossreactivity to beta 1 or beta 3. Recognizes an epitope on the cytoplasmic domain of the protein, and it is thus intracellular. Species crossreactivity: Mouse, hamster and rat.
• Purity/Purification: Serum; Whole rabbit serum.
• Form/Format: Supplied as a liquid with 0.05% sodium azide.

• Applicable Applications for anti-Itgb5 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Radioimmunoassay (RIA)
• Application Notes: Suitable for use in ELISA, RIA, Immunoprecipitation, Western Blot, Immunofluorescence and Immunohistochemistry.; Dilution: Immunoprecipitation: 5uL of antibody is sufficient to precipitate alpha V/beta 5 from 2.5x10e6 cells.; Western Blot: 1:100.; Immunofluorescence: 1:100.; Immunohistochemistry (Acetone Fixed): 1:40-1:80; Immunohistochemistry (Paraffin Embedded): 1:200 (ABC)
• Immunogen: Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human integrin beta 5 subunit (NH2-KPISTHTVDFTFNKFNKSYNGTVD-COOH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-Itgb5 antibody

Antibodies; Abs to Integrin

NCBI and Uniprot Product Information

• NCBI GI #: 225007621
• NCBI GeneID: 16419
• NCBI Accession #: NP_001139356.1
• NCBI GenBank Nucleotide #: NM_001145884.1
• UniProt Accession #: Q6PE70; O70309
• Molecular Weight: 88,052 Da
• NCBI Official Full Name: integrin beta-5 isoform 1
• NCBI Official Synonym Full Names: integrin beta 5
• NCBI Official Symbol: Itgb5
• NCBI Official Synonym Symbols: [b]5; [b]-5; [b]5A; [b]5B; beta5; ESTM23; beta-5; AA475909; AI874634
• NCBI Protein Information: integrin beta-5
• UniProt Protein Name: Integrin beta
• Protein Family: Integrin
• UniProt Gene Name: Itgb5
• UniProt Entry Name: Q6PE70_MOUSE

Uniprot Description

ITGB5: Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand. Heterodimer of an alpha and a beta subunit. Beta-5 associates with alpha-V. Interacts with MYO10. Belongs to the integrin beta chain family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Cellular Component: cell surface; focal adhesion; membrane; leading edge; integral to membrane; integrin complex; receptor complex

Molecular Function: integrin binding; protein binding; receptor activity; receptor binding

Biological Process: integrin-mediated signaling pathway; cell-matrix adhesion; multicellular organismal development; transforming growth factor beta receptor signaling pathway; stress fiber formation; cell adhesion