Mouse Integrin alphaV, beta5 Monoclonal Antibody | anti-ITGB5 antibody

Integrin alphaV, beta5 (Azide Free)

Cat. Num. AAA601769

• Reactivity: Chicken, Human
• Applications: ELISA, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Integrin alphaV; beta5; Monoclonal Antibody; beta5 (Azide Free); Anti -Integrin alphaV; anti-ITGB5 antibody

• Host: Mouse
• Reactivity: Chicken, Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2Q1009
• Specificity: Recognizes human integrin aVb5. Species Crossreactivity: chicken.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS. No preservative added.

• Applicable Applications for anti-ITGB5 antibody: ELISA (EL/EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in the inhibition of attachment of HT29 cells to vitronectin, Inhibition of attachment of carcinoma and melanoma cells to vitronectin, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, Flow Cytometry and ELISA.; Dilution: Inhibition of attachment of HT29 cells to vitronectin: >1:1000; Immunocytochemistry (acetone fixed only): 1:1500 For use on human carcinomas, normal epithelium and keratinocytes.; Immunohistochemistry: Acetone or paraformaldehyde fixed tissues. Optimal dilution determined by the researcher.
• Immunogen: Human UCLA P3 cells (lung carcinoma).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed THP-1 cells showing membrane and cell junction localization.)

Product Categories/Family for anti-ITGB5 antibody

Antibodies; Abs to Integrin

NCBI and Uniprot Product Information

• NCBI GI #: 20127446
• NCBI GeneID: 3693
• NCBI Accession #: NP_002204.2
• NCBI GenBank Nucleotide #: NM_002213.3
• UniProt Accession #: P18084; B0LPF8; B2RD70
• Molecular Weight: 88,054 Da
• NCBI Official Full Name: integrin beta-5
• NCBI Official Synonym Full Names: integrin, beta 5
• NCBI Official Symbol: ITGB5
• NCBI Protein Information: integrin beta-5
• UniProt Protein Name: Integrin beta-5
• Protein Family: Integrin
• UniProt Gene Name: ITGB5
• UniProt Entry Name: ITB5_HUMAN

Uniprot Description

ITGB5: Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand. Heterodimer of an alpha and a beta subunit. Beta-5 associates with alpha-V. Interacts with MYO10. Belongs to the integrin beta chain family.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q21.2

Cellular Component: focal adhesion; cell surface; leading edge; plasma membrane; phagocytic vesicle; receptor complex

Molecular Function: integrin binding; protein binding; receptor activity

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; antigen processing and presentation of peptide antigen via MHC class I; muscle contraction; transforming growth factor beta receptor signaling pathway; cell-matrix adhesion; stress fiber formation; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I