Rabbit anti-Rat Insulin Regulated Aminopeptidase Polyclonal Antibody | anti-LNPEP antibody
Insulin Regulated Aminopeptidase (IRAP, Placental Leucine Aminopeptidase, PLAP, Leucyl Cystinyl Aminopeptidase, LNPEP, Oxytocinase, Otase, Vesicle Protein of 165kD, vp165)
Gene Names
LNPEP; IRAP
Reactivity
Rat
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Insulin Regulated Aminopeptidase; Polyclonal Antibody; Insulin Regulated Aminopeptidase (IRAP; Placental Leucine Aminopeptidase; PLAP; Leucyl Cystinyl Aminopeptidase; LNPEP; Oxytocinase; Otase; Vesicle Protein of 165kD; vp165); Anti -Insulin Regulated Aminopeptidase (IRAP; anti-LNPEP antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat IRAP. Species sequence homology: mouse-82% and human (isoforms 1 and 2)-76%.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.1% BSA, 40% glycerol.
Applicable Applications for anti-LNPEP antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.
Dilution: Western Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.
Immunogen
Synthetic 17 amino acid peptide sequence from rat IRAP extracellular domain (KLH coupled).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-LNPEP antibody
IRAP (also known as placental leucine aminopeptidase, PLAP; leucyl cystinyl aminopeptidase, LNPEP; oxytocinase, Otase; vesicle protein of 165kD or vp165) belongs to the peptidase M1 family. IRAP (mouse 966aa, rat 1025 aa; human 1025 aa, chromosome 5q14.2-q15), a zinc-containing metalloprotein, degrades peptide hormones such as oxytocin, vasopressin, and angiotensin III, and inactivates Met-enkephalin and dynorphin. It is a type 2 membrane protein and is secreted due to proteolytic processing. IRAP is glycosylated and alternatively spliced to at least 3 isoform (1-15 aa missing in isoform 2 and 1-20 missing in isoform 3). The N-terminus (1-110 aa) is located in the cytoplasm, followed by TM domain (111-131 aa); the C-terminus (132-1025 aa) is extracellular. The secreted form is cleaved at 154-155 aa (processed secreted form 155-1025 aa). IRAP is highly expressed in placenta, heart, kidney, and small intestine. In brain, only the membrane form is found. The brain form is found to be 140kD due to differential glycosylation.
Product Categories/Family for anti-LNPEP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
117,368 Da
NCBI Official Full Name
insulin-regulated aminopeptidase
NCBI Official Symbol
LNPEP
NCBI Official Synonym Symbols
IRAP
NCBI Protein Information
leucyl-cystinyl aminopeptidase; insulin-regulated aminopeptidase
UniProt Protein Name
Insulin-regulated aminopeptidase
Protein Family
UniProt Gene Name
IRAP
UniProt Entry Name
A7WK50_PIG
Similar Products
Product Notes
The LNPEP irap (Catalog #AAA618570) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Insulin Regulated Aminopeptidase (IRAP, Placental Leucine Aminopeptidase, PLAP, Leucyl Cystinyl Aminopeptidase, LNPEP, Oxytocinase, Otase, Vesicle Protein of 165kD, vp165) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Insulin Regulated Aminopeptidase can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1-10ug/ml using ECL. ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml. Researchers should empirically determine the suitability of the LNPEP irap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Insulin Regulated Aminopeptidase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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