Human High Sensitive Leucyl/Cystinyl Aminopeptidase (LNPEP) ELISA Kit | LNPEP elisa kit

Human High Sensitive Leucyl/Cystinyl Aminopeptidase (LNPEP) ELISA Kit

Cat. Num. AAA2122215

• Gene Names: LNPEP; CAP; IRAP; PLAP; P-LAP
• Reactivity: Human
• Synonyms: High Sensitive Leucyl/Cystinyl Aminopeptidase (LNPEP); Human High Sensitive Leucyl/Cystinyl Aminopeptidase (LNPEP) ELISA Kit; CAP; PLAP; OTASE; IRAP; Oxytocinase; Insulin-regulated membrane aminopeptidase; Insulin-responsive aminopeptidase; Placental leucine aminopeptidase; LNPEP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Leucyl/Cystinyl Aminopeptidase (LNPEP).; No significant cross-reactivity or interference between High Sensitive Leucyl/Cystinyl Aminopeptidase (LNPEP) and analogues was observed.
• Sequence Length: 1025

• Assay Type: Double-antibody Sandwich
• Sample: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Dectection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.060ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Leucyl/Cystinyl Aminopeptidase (LNPEP) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Leucyl/Cystinyl Aminopeptidase (LNPEP) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LNPEP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Leucyl/Cystinyl Aminopeptidase (LNPEP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Leucyl/Cystinyl Aminopeptidase (LNPEP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Leucyl/Cystinyl Aminopeptidase (LNPEP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Leucyl/Cystinyl Aminopeptidase (LNPEP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for LNPEP elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 61742777
• NCBI GeneID: 4012
• NCBI Accession #: NP_005566.2
• NCBI GenBank Nucleotide #: NP_005566.2
• UniProt Accession #: Q9UIQ6
• NCBI Official Full Name: leucyl-cystinyl aminopeptidase isoform 1
• NCBI Official Synonym Full Names: leucyl and cystinyl aminopeptidase
• NCBI Official Symbol: LNPEP
• NCBI Official Synonym Symbols: CAP; IRAP; PLAP; P-LAP
• NCBI Protein Information: leucyl-cystinyl aminopeptidase
• UniProt Protein Name: Leucyl-cystinyl aminopeptidase
• Protein Family: Leucyl-cystinyl aminopeptidase
• UniProt Gene Name: LNPEP
• UniProt Synonym Gene Names: OTASE; Cystinyl aminopeptidase; IRAP; OTase; P-LAP
• UniProt Entry Name: LCAP_HUMAN

NCBI Description

This gene encodes a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin, dynorphin A and other peptide hormones. The protein can be secreted in maternal serum, reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4, or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

IRAP: a leucyl/cystinyl aminopeptidase. A type II membrane protein and secreted. Resides in intracellular vesicles together with GLUT4 and can then translocate to the cell surface in response to insulin and/or oxytocin. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain. Three alternatively spliced isoforms have been described.

Protein type: EC 3.4.11.3; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q15

Cellular Component: cytoplasmic vesicle membrane; membrane; perinuclear region of cytoplasm; lysosomal membrane; integral to plasma membrane; extracellular region; plasma membrane; intracellular; cytosol

Molecular Function: zinc ion binding; metallopeptidase activity; aminopeptidase activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; protein polyubiquitination; cell-cell signaling; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein catabolic process; female pregnancy; proteolysis