Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using INSR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Rabbit Insulin Receptor beta Polyclonal Antibody | anti-INSR antibody
INSR Polyclonal Antibody
Gene Names
INSR; HHF5; CD220
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunoprecipitation
Purity
Affinity purification
Synonyms
Insulin Receptor beta; Polyclonal Antibody; INSR Polyclonal Antibody; INSR; Insulin Receptor beta ; Insulin Receptor b ; CD220; HHF5 ;; anti-INSR antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
1382
Applicable Applications for anti-INSR antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western Blot (WB): 1:500-1: 2000
Immunocytochemistry (ICC): 1:50-1: 200
Immunoprecipitation (IP): 1:20-1: 50
Immunocytochemistry (ICC): 1:50-1: 200
Immunoprecipitation (IP): 1:20-1: 50
Immunogen
N term -peptide of human INSR
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C or -80 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using INSR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using INSR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse liver using INSR antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse liver using INSR antibody at dilution of 1:100 (40x lens).)
Related Product Information for anti-INSR antibody
Western blot analysis of Hela cell lysate using INSR antibody.
References
1. Adams, T.E. et al. (2000) Cell. Mol. Life Sci. 57, 10501093. 2. Baserga, R. et al. (2000) Oncogene 19, 55745581. 3. Scheidegger, K.J. et al. (2000) J. Biol. Chem. 275, 3892138928. 4. HernandezSanchez, C. et al. (1995) J. Biol. Chem. 270, 2917629181. 5. Lopaczynski, W. et al. (2000) Biochem. Biophys. Res. Commun. 279, 955960. 6. Baserga, R. et al. (1999) Exp. Cell Res. 253, 16. 7. White, M.F. et al. (1985) J. Biol. Chem. 260, 94709478. 8. White, M.F. et al. (1988) J. Biol. Chem. 263, 29692980.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
156.35kDa
NCBI Official Full Name
insulin receptor isoform Long preproprotein
NCBI Official Synonym Full Names
insulin receptor
NCBI Official Symbol
INSR
NCBI Official Synonym Symbols
HHF5; CD220
NCBI Protein Information
insulin receptor; IR
UniProt Protein Name
Insulin receptor
Protein Family
UniProt Gene Name
INSR
UniProt Synonym Gene Names
IR
UniProt Entry Name
INSR_HUMAN
Similar Products
Product Notes
The INSR insr (Catalog #AAA125375) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The INSR Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Insulin Receptor beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot (WB): 1:500-1: 2000 Immunocytochemistry (ICC): 1:50-1: 200 Immunoprecipitation (IP): 1:20-1: 50. Researchers should empirically determine the suitability of the INSR insr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Insulin Receptor beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
