Human Major Histocompatibility Complex Class I B (MHCB) ELISA Kit | MHCB elisa kit

Human Major Histocompatibility Complex Class I B (MHCB) ELISA Kit

Cat. Num. AAA2000223

• Gene Names: HLA-B; AS; HLAB; SPDA1
• Reactivity: Human
• Synonyms: Major Histocompatibility Complex Class I B (MHCB); Human Major Histocompatibility Complex Class I B (MHCB) ELISA Kit; MHC-B; HLA-B; HLAB; AS; SPDA1; Leukocyte Antigen B; Ankylosing Spondylitis; HLA class I histocompatibility antigen; B-7 alpha chain; MHCB elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Major Histocompatibility Complex Class I B (MHCB).; No significant cross-reactivity or interference between Major Histocompatibility Complex Class I B (MHCB) and analogues was observed.
• Sequence Length: 362

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Major Histocompatibility Complex Class I B (MHCB) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Major Histocompatibility Complex Class I B (MHCB) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MHCB elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class I B (MHCB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class I B (MHCB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class I B (MHCB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Major Histocompatibility Complex Class I B (MHCB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MHCB elisa kit

Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 17986001
• NCBI GeneID: 3106
• NCBI Accession #: NP_005505.2
• NCBI GenBank Nucleotide #: NM_005514.6
• UniProt Accession #: P01889; Q29638; Q29681; Q29854; Q29861; Q31613; Q5SRJ2; Q9GIX1; Q9TP95; P03989; P10319; P18463; P18464; P18465; P30460; P30461; P30462; P30464
• Molecular Weight: 40,460 Da
• NCBI Official Full Name: major histocompatibility complex, class I, B
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, B
• NCBI Official Symbol: HLA-B
• NCBI Official Synonym Symbols: AS; HLAB; SPDA1
• NCBI Protein Information: major histocompatibility complex, class I, B
• UniProt Protein Name: HLA class I histocompatibility antigen, B-7 alpha chain
• UniProt Gene Name: HLA-B
• UniProt Synonym Gene Names: HLAB
• UniProt Entry Name: 1B07_HUMAN

NCBI Description

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

HLAB iso7: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; membrane; integral to plasma membrane; early endosome membrane; endoplasmic reticulum; plasma membrane; integral to membrane; MHC class I protein complex

Molecular Function: peptide antigen binding; chaperone binding; TAP binding; receptor binding

Biological Process: regulation of immune response; viral reproduction; detection of bacterium; regulation of interleukin-12 production; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; defense response; protection from natural killer cell mediated cytotoxicity; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; regulation of T cell anergy; regulation of interleukin-6 production; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; immune response