Rabbit anti-Human Inhibin beta B Polyclonal Antibody | anti-INHBB antibody

Anti-Inhibin beta B Antibody, Rabbit Polyclonal

Cat. Num. AAA8101218

• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: Inhibin beta B; Polyclonal Antibody; Anti-Inhibin beta B Antibody; Rabbit Polyclonal; Inhibin beta B/INHBB Antibody; Rabbit PAb; Antigen Affinity Purified; inhibin beta B; Anti-INHBB Antibody; Anti-MGC157939 Antibody; anti-INHBB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Inhibin beta B/INHBB
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-INHBB antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:100-1:500; ICC/IF: 1:100-1:500
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human Inhibin beta B/INHBB
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Inhibin beta B/INHBB, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human Inhibin beta B in human liver with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human Inhibin beta B in mouse liver with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of Inhibin beta B in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human Inhibin beta B polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Related Product Information for anti-INHBB antibody

Activin and inhibin are two closely related protein complexes that have almost directly opposite biological effects. The activin and inhibin protein complexes are both dimeric in structure, and, in each complex, the two monomers are linked to one another by a single disulfide bond. Activin is composed of two beta subunits, betaA betaA (activin A), betaB betaB (activin B), or betaA betaB (activin AB). Inhibin is composed of an alpha and one of two beta subunits, betaA (inhibin A) or betaB (inhibin B). Activins are produced in many cell types and organs, such as gonads, pituitary gland, and placenta. In the ovarian follicle, activin increases FSH binding and FSH-induced aromatization. It participates in androgen synthesis enhancing LH action in the ovary and testis. In the male, activin enhances spermatogenesis. Also, Activin plays a role in wound repair and skin morphogenesis. Activin is strongly expressed in wounded skin, and overexpression of activin in the epidermis of transgenic mice improves wound healing and enhances scar formation. Activin also regulates the morphogenesis of branching organs such as the prostate, lung, and kidney. There is also evidence showed that lack of activin during development results in neural developmental defects.

Product Categories/Family for anti-INHBB antibody

Early Mesodermal Lineage Markers

References

Feng ZM, et al. (1989) Characterization and regulation of testicular inhibin beta-subunit mRNA. Mol Endocrinol. 3 (6): 939-48.Bernard DJ, et al. (2002) Inhibin binding protein (InhBP/p120), betaglycan, and the continuing search for the inhibin receptor. Mol Endocrinol. 16 (2): 207-12.Lejeune H, et al. (1997) Stimulating effect of both human recombinant inhibin A and activin A on immature porcine Leydig cell functions in vitro. Endocrinology. 138 (11): 4783-91.

NCBI and Uniprot Product Information

• NCBI GI #: 154813204
• NCBI GeneID: 3625
• NCBI Accession #: NP_002184.2
• NCBI GenBank Nucleotide #: NM_002193.2
• UniProt Accession #: P09529; Q53T31; Q8N1D3
• Molecular Weight: 45,122 Da
• NCBI Official Full Name: inhibin beta B chain preproprotein
• NCBI Official Synonym Full Names: inhibin, beta B
• NCBI Official Symbol: INHBB
• NCBI Protein Information: inhibin beta B chain; Inhibin, beta-2; activin beta-B chain; activin AB beta polypeptide
• UniProt Protein Name: Inhibin beta B chain
• Protein Family: Inhibin
• UniProt Gene Name: INHBB
• UniProt Entry Name: INHBB_HUMAN

NCBI Description

The inhibin beta B subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumour-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta B subunit forms a homodimer, activin B, and also joins with the beta A subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. [provided by RefSeq, Jul 2008]

Uniprot Description

INHBB: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 2cen-q13

Cellular Component: extracellular space; perinuclear region of cytoplasm; extracellular region

Molecular Function: protein binding; protein homodimerization activity; growth factor activity; hormone activity; cytokine activity; transforming growth factor beta receptor binding; host cell surface receptor binding

Biological Process: fat cell differentiation; activin receptor signaling pathway; defense response; negative regulation of hepatocyte growth factor biosynthetic process; cellular response to starvation; negative regulation of insulin secretion; negative regulation of follicle-stimulating hormone secretion; regulation of apoptosis; cellular response to insulin stimulus; ovarian follicle development; response to mechanical stimulus; regulation of MAPKKK cascade; positive regulation of follicle-stimulating hormone secretion; cell differentiation; cell development; oocyte development; growth