Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with POLR2C Antibody (C-term). MBS9208958 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Rabbit POLR2C Polyclonal Antibody | anti-POLR2C antibody
POLR2C Antibody (C-term)
WB~~1:1000
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with POLR2C Antibody (C-term). MBS9208958 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with POLR2C Antibody (C-term). MBS9208958 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Western Blot (WB)
(POLR2C Antibody (C-term) western blot analysis in Hela,U251 cell line and mouse skeletal muscle tissue lysates (35ug/lane).This demonstrates the POLR2C antibody detected the POLR2C protein (arrow).)
Western Blot (WB)
(POLR2C Antibody (C-term) western blot analysis in Hela,U251 cell line and mouse skeletal muscle tissue lysates (35ug/lane).This demonstrates the POLR2C antibody detected the POLR2C protein (arrow).)
Dammann R., et al. Biochim. Biophys. Acta 1396:153-157(1998).
Bruno T., et al. Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
Loftus B.J., et al. Genomics 60:295-308(1999).
Bienvenut W.V., et al. Submitted (MAR-2009) to UniProtKB.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21. [provided by RefSeq, Jul 2008]
Uniprot Description
POLR2C: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft. Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family.
Protein type: Nucleotide Metabolism - pyrimidine; DNA-binding; Transcription initiation complex; Nucleotide Metabolism - purine
Chromosomal Location of Human Ortholog: 16q13-q21
Cellular Component: nucleoplasm; microtubule cytoskeleton; cytoplasm; DNA-directed RNA polymerase II, core complex; nucleus
Molecular Function: protein dimerization activity; DNA binding
Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; somatic stem cell maintenance; nucleotide-excision repair; positive regulation of viral transcription; RNA splicing; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; gene expression; DNA repair
Research Articles on POLR2C
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Product Notes
The POLR2C polr2c (Catalog #AAA9208958) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 200-228. The POLR2C Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's POLR2C can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB). IF~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the POLR2C polr2c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLR2C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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