Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Sorbitol dehydrogenase (SDH) Standard Curve.)
Rat Sorbitol Dehydrogenase (SDH) ELISA Kit | SDH elisa kit
Rat Sorbitol Dehydrogenase (SDH) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Sorbitol dehydrogenase (SDH) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Sorbitol dehydrogenase (SDH) Standard Curve.)
Background: Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]
NCBI and Uniprot Product Information
NCBI Description
catalyzes the conversion of L-iditol and NAD+ to L-sorbose and NADH in the polyol pathway; acts as a tetramer with one zinc atom per subunit [RGD, Feb 2006]
Uniprot Description
SORD: Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. Belongs to the zinc-containing alcohol dehydrogenase family.
Protein type: EC 1.1.1.14; Carbohydrate Metabolism - fructose and mannose; Oxidoreductase
Cellular Component: mitochondrion; membrane; mitochondrial membrane
Molecular Function: identical protein binding; zinc ion binding; L-iditol 2-dehydrogenase activity; NAD binding
Biological Process: response to drug; response to nutrient levels; sperm motility; response to cadmium ion; L-xylitol metabolic process; response to copper ion; sorbitol metabolic process; L-xylitol catabolic process; response to hormone stimulus; fructose biosynthetic process; response to osmotic stress; sorbitol catabolic process
Research Articles on SDH
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Product Notes
The Rat SDH sord (Catalog #AAA9711848) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711848 ELISA Kit recognizes Rat SDH. It is sometimes possible for the material contained within the vial of "Sorbitol Dehydrogenase (SDH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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