Inhibin beta A/Activin A Active Protein | INHBA active protein

Inhibin beta A/Activin A Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122899

• Purity: >88% as determined by SDS-PAGE
• Synonyms: Inhibin beta A/Activin A; Inhibin beta A/Activin A Protein; Mouse; Recombinant (His Tag); Mouse Inhibin beta A/INHBA/Activin A Protein (His Tag); inhibin; beta A; INHBA active protein

• Host: HEK293 Cells
• Purity/Purification: >88% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser424

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the pro form of mouse INHBA (NP_032406.1)(Met 1-Ser 424) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its binding ability in a functional ELISA.; 2. Immobilized human ACVR2B at 10ug/mL (100uL/well) can bind biotinylated mouse INHBA-His. The EC50 of biotinylated mouse INHBA-His is 0.161ug/mL.; 3. Immobilized mouse INHBA-his at 10ug/mL (100uL/well) can bind human Follistatin Protein. The EC50 of human Follistatin Protein is 0.39ug/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. 1. Immobilized human ACVR2B at 10 ug/mL (100 ul/well) can bind biotinylated mouse INHBA-His, The EC50 of biotinylated mouse INHBA-His is 0.161 ug/mL. 2. Immobilized mouse INHBA-his at 10 ug/mL (100 ul/well) can bind human Follistatin Protein, The EC50 of human Follistatin Protein is 0.39 ug/mL.)

Related Product Information for INHBA active protein

Background: Activin and inhibin are two closely related protein complexes that have almost directly opposite biological effects. The activin and inhibin protein complexes are both dimeric in structure, and, in each complex, the two monomers are linked to one another by a single disulfide bond. Activin is composed of two beta subunits, betaA betaA (activin A), betaB betaB (activin B), or betaA betaB (activin AB). Inhibin is composed of an alpha and one of two beta subunits, betaA (inhibin A) or betaB (inhibin B). Activins are produced in many cell types and organs, such as gonads, pituitary gland, and placenta. In the ovarian follicle, activin increases FSH binding and FSH-induced aromatization. It participates in androgen synthesis enhancing LH action in the ovary and testis. In the male, activin enhances spermatogenesis. Also, Activin plays a role in wound repair and skin morphogenesis. Activin is strongly expressed in wounded skin, and overexpression of activin in the epidermis of transgenic mice improves wound healing and enhances scar formation. Activin also regulates the morphogenesis of branching organs such as the prostate, lung, and kidney. There is also evidence showed that lack of activin during development results in neural developmental defects.

Product Categories/Family for INHBA active protein

Early Mesodermal Lineage Markers

References

Tanimoto K, et al. (1992) Structure and sequence analysis of the human activin beta A subunit gene. DNA Seq. 2 (2): 103-10.Welt C, et al. (2002) Activins, inhibins, and follistatins: from endocrinology to signaling. A paradigm for the new millennium. Exp Biol Med. 227 (9): 724-52.Xu J, et al. (1995) Inhibin antagonizes inhibition of liver cell growth by activin by a dominant-negative mechanism. J Biol Chem. 270 (11): 6308-13.

NCBI and Uniprot Product Information

• Molecular Weight: The secreted recombinant mouse INHBA comprises 415 amino acids and has a calculated molecular mass of 46.5kDa. As a result of glycosylation, the apparent molecular mass of rmINHBA is approximately 18 & 43kDa corresponding to the mature inhibin beta A chai
• Protein Family: Inhibin