Rabbit anti-Human ING5 Polyclonal Antibody | anti-ING5 antibody

ING5, ID (ING5, Inhibitor of growth protein 5, p28ING5) (Biotin)

Cat. Num. AAA6310533

• Gene Names: ING5; p28ING5
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ING5; Polyclonal Antibody; ID (ING5; Inhibitor of growth protein 5; p28ING5) (Biotin); p28ING5; anti-ING5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ING5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ING5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98~127 amino acids from the Central region of human ING5.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ING5 Antibody (Center) in A2058 cell line lysates (35ug/lane). ING5 (arrow) was detected using the purified Pab.)

Related Product Information for anti-ING5 antibody

ING5 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway. [provided by RefSeq].

Product Categories/Family for anti-ING5 antibody

Antibodies; Tumor Suppressors

References

Ullah, M., et al. Mol. Cell. Biol. 28(22):6828-6843(2008). Champagne, K.S., et al. Proteins 72(4):1371-1376(2008). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 57012960
• NCBI GeneID: 84289
• UniProt Accession #: Q8WYH8; Q53NU6; Q57Z54; Q9BS30; A8K1P3
• Molecular Weight: 240
• NCBI Official Full Name: Inhibitor of growth protein 5
• NCBI Official Synonym Full Names: inhibitor of growth family, member 5
• NCBI Official Symbol: ING5
• NCBI Official Synonym Symbols: p28ING5
• NCBI Protein Information: inhibitor of growth protein 5
• UniProt Protein Name: Inhibitor of growth protein 5
• Protein Family: Inhibitor of growth protein
• UniProt Gene Name: ING5
• UniProt Entry Name: ING5_HUMAN

NCBI Description

The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

ING5: Component of the HBO1 complex which has a histone H4- specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator. Belongs to the ING family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; zinc ion binding; chromatin binding; methylated histone residue binding

Biological Process: negative regulation of cell proliferation; establishment and/or maintenance of chromatin architecture; protein amino acid acetylation; transcription, DNA-dependent; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; DNA replication; negative regulation of growth