Mouse CD31/PECAM-1 Monoclonal Antibody | anti-PECAM1 antibody

CD31/PECAM-1 (Endothelial Cell Marker)

Cat. Num. AAA4381159

• Gene Names: PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
• Reactivity: Human, Monkey, Rabbit
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified Ab conjugated to CF488
• Synonyms: CD31/PECAM-1; Monoclonal Antibody; CD31/PECAM-1 (Endothelial Cell Marker); EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA'; anti-PECAM1 antibody

• Host: Mouse
• Reactivity: Human, Monkey, Rabbit
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: C31.7
• Specificity: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
• Purity/Purification: Purified Ab conjugated to CF488
• Form/Format: Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.
• Sequence Length: 738

• Applicable Applications for anti-PECAM1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: FC/FACS: 5ul per test per one million cells or 5ul per 100ul of whole blood; IF: 1:50-1:100
• Positive Control: Jurkat, CCRF-CEM or K-562 cells. Tonsil, Angiosarcoma.
• Immunogen: Recombinant human CD31 protein
• Cellular Localization: Cell Surface and Cytoplasmic
• Hu-chromosome Location: 17q23.3
• Preparation and Storage: Antibody with azide: store at 4 to 8 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Flow Cytometry (FC/FACS)

(Flow Cytometry of human CD31 on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled CD31 Monoclonal Antibody (C31.7).)

References

Gratzinger D et. al. Am J Clin Pathol 131:264-278 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 313760624
• NCBI GeneID: 5175
• NCBI Accession #: NP_000433.4
• NCBI GenBank Nucleotide #: NP_000433.4
• UniProt Accession #: P16284
• Molecular Weight: ~100kDa (endothelium) and ~130kDa (platelets)
• NCBI Official Full Name: platelet endothelial cell adhesion molecule
• NCBI Official Synonym Full Names: platelet and endothelial cell adhesion molecule 1
• NCBI Official Symbol: PECAM1
• NCBI Official Synonym Symbols: CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
• NCBI Protein Information: platelet endothelial cell adhesion molecule
• UniProt Protein Name: Platelet endothelial cell adhesion molecule
• Protein Family: Platelet endothelial cell adhesion molecule
• UniProt Gene Name: PECAM1
• UniProt Synonym Gene Names: PECAM-1
• UniProt Entry Name: PECA1_HUMAN

NCBI Description

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

Uniprot Description

PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: extracellular space; platelet alpha granule membrane; integral to membrane; plasma membrane; cell junction

Molecular Function: protein binding

Biological Process: platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; diapedesis; cell recognition; blood coagulation; signal transduction; cell adhesion; leukocyte migration; phagocytosis