Rabbit anti-Human, Mouse INF2 Polyclonal Antibody | anti-INF2 antibody

INF2, ID (INF2, C14orf151, C14orf173, Inverted formin-2, HBEBP2-binding protein C) (PE)

Cat. Num. AAA6310508

• Gene Names: INF2; FSGS5; CMTDIE; pp9484; C14orf151; C14orf173
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: INF2; Polyclonal Antibody; ID (INF2; C14orf151; C14orf173; Inverted formin-2; HBEBP2-binding protein C) (PE); HBEBP2-binding protein C; anti-INF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-INF2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: INF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 588-616 amino acids from the Central region of human INF2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(INF2 Antibody (Center) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the INF2 antibody detected the INF2 protein (arrow).)

Related Product Information for anti-INF2 antibody

This gene represents a member of the formin family of proteins. It is considered a diaphanous formin due to the presence of a diaphanous inhibitory domain located at the N-terminus of the encoded protein. Studies of a similar mouse protein indicate that the protein encoded by this locus may function in polymerization and depolymerization of actin filaments. Mutations at this locus have been associated with focal segmental glomerulosclerosis 5.

Product Categories/Family for anti-INF2 antibody

Antibodies; Binding Proteins

References

Brown, E.J., et al. Nat. Genet. 42(1):72-76(2010). Chhabra, E.S., et al. J. Biol. Chem. 281(36):26754-26767(2006). Bindschadler, M., et al. Proc. Natl. Acad. Sci. U.S.A. 101(41):14685-14686(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 149999378
• NCBI GeneID: 64423
• NCBI Accession #: NP_001026884.3
• NCBI GenBank Nucleotide #: NM_001031714.3
• UniProt Accession #: Q27J81; Q27J83; Q69YL8; Q6P1X7; Q6PK22; Q86TR7; Q9BRM1; Q9H6N1
• Molecular Weight: 135,624 Da
• NCBI Official Full Name: inverted formin-2 isoform 2
• NCBI Official Synonym Full Names: inverted formin, FH2 and WH2 domain containing
• NCBI Official Symbol: INF2
• NCBI Official Synonym Symbols: FSGS5; CMTDIE; pp9484; C14orf151; C14orf173
• NCBI Protein Information: inverted formin-2; HBEBP2-binding protein C; HBEAG-binding protein 2 binding protein C
• UniProt Protein Name: Inverted formin-2
• Protein Family: Inverted formin
• UniProt Gene Name: INF2
• UniProt Synonym Gene Names: C14orf151; C14orf173

Uniprot Description

INF2: Severs actin filaments and accelerates their polymerization and depolymerization. Defects in INF2 are the cause of focal segmental glomerulosclerosis type 5 (FSGS5). A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. Defects in INF2 are the cause of Charcot-Marie-Tooth disease, dominant intermediate type E (CMTDIE). A form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. The dominant intermediate type E is characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. Patients additionally manifest focal segmental glomerulonephritis, proteinuria, progression to end- stage renal disease, and a characteristic histologic pattern on renal biopsy. Belongs to the formin homology family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 14q32.33

Cellular Component: perinuclear region of cytoplasm

Disease: Charcot-marie-tooth Disease, Dominant Intermediate E; Focal Segmental Glomerulosclerosis 5