Human RCC1 And BTB Domain Containing Protein 2 (RCBTB2) ELISA Kit | RCBTB2 elisa kit

Human RCC1 And BTB Domain Containing Protein 2 (RCBTB2) ELISA Kit

Cat. Num. AAA2707047

• Gene Names: RCBTB2; RLG; CHC1L
• Reactivity: Human
• Synonyms: RCC1 And BTB Domain Containing Protein 2 (RCBTB2); Human RCC1 And BTB Domain Containing Protein 2 (RCBTB2) ELISA Kit; CHC1L; RLG; RCC1-like G exchanging factor; Regulator Of Chromosome Condensation And BTB(POZ)Domain Containing Protein 2; Chromosome Condensation 1-Like; RCBTB2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RCC1 And BTB Domain Containing Protein 2 (RCBTB2).; No significant cross-reactivity or interference between RCC1 And BTB Domain Containing Protein 2 (RCBTB2) and analogues was observed.
• Sequence Length: 527

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.124ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RCC1 And BTB Domain Containing Protein 2 (RCBTB2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RCC1 And BTB Domain Containing Protein 2 (RCBTB2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RCBTB2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RCC1 And BTB Domain Containing Protein 2 (RCBTB2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RCC1 And BTB Domain Containing Protein 2 (RCBTB2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RCC1 And BTB Domain Containing Protein 2 (RCBTB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RCC1 And BTB Domain Containing Protein 2 (RCBTB2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557445
• NCBI GeneID: 1102
• NCBI Accession #: NP_001259.1
• NCBI GenBank Nucleotide #: NM_001268.3
• UniProt Accession #: O95199; B2RDW8
• Molecular Weight: 57,769 Da
• NCBI Official Full Name: RCC1 and BTB domain-containing protein 2 isoform 2
• NCBI Official Synonym Full Names: RCC1 and BTB domain containing protein 2
• NCBI Official Symbol: RCBTB2
• NCBI Official Synonym Symbols: RLG; CHC1L
• NCBI Protein Information: RCC1 and BTB domain-containing protein 2
• UniProt Protein Name: RCC1 and BTB domain-containing protein 2
• Protein Family: RCC1 and BTB domain-containing protein
• UniProt Gene Name: RCBTB2
• UniProt Synonym Gene Names: CHC1L; RLG; CHC1-L

NCBI Description

This gene encodes a protein containing two C-terminal BTB/POZ domains that is related to regulator of chromosome condensation (RCC). The encoded protein may act as a guanine nucleotide exchange factor. This gene is observed to be lost or underexpressed in prostate cancers. There is a pseudogene of this gene on chromosome 10. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]