Rabbit anti-Human IL5RA Polyclonal Antibody | anti-IL5RA antibody

IL5RA, CT (IL5RA, IL5R, Interleukin-5 receptor subunit alpha, CDw125, CD125)

Cat. Num. AAA6000846

• Gene Names: IL5RA; IL5R; CD125; CDw125; HSIL5R3
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: IL5RA; Polyclonal Antibody; CT (IL5RA; IL5R; Interleukin-5 receptor subunit alpha; CDw125; CD125); Anti -IL5RA; anti-IL5RA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-IL5RA antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: IL5RA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-420 amino acids from the C-terminal region of human IL5RA.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(IL5RA Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the IL5RA antibody detected the IL5RA protein (arrow).)

Related Product Information for anti-IL5RA antibody

The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Six alternatively spliced transcript variants encoding three distinct isoforms have been reported.

Product Categories/Family for anti-IL5RA antibody

Antibodies; Abs to CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 340745316
• NCBI GeneID: 3568
• NCBI Accession #: NP_001230028.1
• NCBI GenBank Nucleotide #: NM_001243099.1
• UniProt Accession #: Q01344; Q14633; Q15469; Q6ISX9; B3IU77; B4E2G0
• Molecular Weight: 47,685 Da
• NCBI Official Full Name: interleukin-5 receptor subunit alpha isoform 4
• NCBI Official Synonym Full Names: interleukin 5 receptor, alpha
• NCBI Official Symbol: IL5RA
• NCBI Official Synonym Symbols: IL5R; CD125; CDw125; HSIL5R3
• NCBI Protein Information: interleukin-5 receptor subunit alpha; CD125 antigen; IL-5R subunit alpha; IL-5 receptor subunit alpha; interleukin 5 receptor type 3; interleukin-5 receptor alpha chain
• UniProt Protein Name: Interleukin-5 receptor subunit alpha
• Protein Family: Interleukin-5 receptor
• UniProt Gene Name: IL5RA
• UniProt Synonym Gene Names: IL5R; IL-5 receptor subunit alpha; IL-5R subunit alpha; IL-5R-alpha; IL-5RA
• UniProt Entry Name: IL5RA_HUMAN

NCBI Description

The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported. [provided by RefSeq, Jul 2011]

Uniprot Description

IL5RA: This is the receptor for interleukin-5. The alpha chain binds to IL5. Belongs to the type I cytokine receptor family. Type 5 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p26-p24

Cellular Component: extracellular space; integral to membrane; plasma membrane

Molecular Function: protein binding; interleukin-5 receptor activity

Biological Process: regulation of interleukin-5 production; cell proliferation; inflammatory response to antigenic stimulus; signal transduction