Rabbit anti-Human ATP6V1A Polyclonal Antibody | anti-ATP6V1A antibody

ATP6V1A, ID (ATP6V1A, ATP6A1, ATP6V1A1, VPP2, V-type proton ATPase catalytic subunit A, V-ATPase 69kD subunit, Vacuolar ATPase isoform VA68, Vacuolar proton pump subunit alpha) (PE)

Cat. Num. AAA6276666

• Gene Names: ATP6V1A; HO68; VA68; VPP2; Vma1; ATP6A1; ATP6V1A1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATP6V1A; Polyclonal Antibody; ID (ATP6V1A; ATP6A1; ATP6V1A1; VPP2; V-type proton ATPase catalytic subunit A; V-ATPase 69kD subunit; Vacuolar ATPase isoform VA68; Vacuolar proton pump subunit alpha) (PE); Vacuolar proton pump subunit alpha; anti-ATP6V1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ATP6V1A antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ATP6V1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 447~477 amino acids from the Central region of human ATP6V1A.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ATP6V1A Antibody (Center) in A2058 cell line lysates (35ug/lane). ATP6V1A (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(ATP6V1A Antibody (Center) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP6V1A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-ATP6V1A antibody

Antibodies; Enzymes; ATPase

References

Martins-de-Souza, D., et al. J Psychiatr Res 43(11):978-986(2009). Martins-de-Souza, D., et al. BMC Psychiatry 9, 17 (2009). Lu, M., et al. J. Biol. Chem. 282(34):24495-24503(2007). Ahmed, M., et al. J. Proteome Res. 4(3):931-940(2005). Morel, N. Biol. Cell 95(7):453-457(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 22096378
• NCBI GeneID: 523
• UniProt Accession #: P38606; Q53YD9; Q96DY6; Q9UHY3; B2RBR8; B7Z1R5; D3DN75
• Molecular Weight: 617
• NCBI Official Full Name: V-type proton ATPase catalytic subunit A
• NCBI Official Synonym Full Names: ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
• NCBI Official Symbol: ATP6V1A
• NCBI Official Synonym Symbols: HO68; VA68; VPP2; Vma1; ATP6A1; ATP6V1A1
• NCBI Protein Information: V-type proton ATPase catalytic subunit A; V-ATPase subunit A; V-ATPase A subunit 1; V-ATPase 69 kDa subunit 1; vacuolar ATPase isoform VA68; vacuolar proton pump subunit alpha; vacuolar proton pump alpha subunit 1; ATPase, H+ transporting, lysosomal, subu
• UniProt Protein Name: V-type proton ATPase catalytic subunit A
• UniProt Gene Name: ATP6V1A
• UniProt Synonym Gene Names: ATP6A1; ATP6V1A1; VPP2; V-ATPase subunit A
• UniProt Entry Name: VATA_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP6V1A: Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the ATPase alpha/beta chains family.

Protein type: EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation; Hydrolase

Chromosomal Location of Human Ortholog: 3q13.31

Cellular Component: microvillus; mitochondrion; integral to plasma membrane; lysosomal membrane; apical plasma membrane; plasma membrane; proton-transporting two-sector ATPase complex; cytosol

Molecular Function: hydrogen ion transporting ATPase activity, rotational mechanism; ATP binding

Biological Process: ATP metabolic process; interaction with host; cellular iron ion homeostasis; transport; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; transmembrane transport