Rabbit anti-Human IL32 Polyclonal Antibody | anti-IL32 antibody

IL32, NT (IL32, NK4, TAIF, Interleukin-32, Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor) (Azide free) (HRP)

Cat. Num. AAA6310227

• Gene Names: IL32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IL32; Polyclonal Antibody; NT (IL32; NK4; TAIF; Interleukin-32; Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor) (Azide free) (HRP); Tumor necrosis factor alpha-inducing factor; anti-IL32 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IL32.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 188

• Applicable Applications for anti-IL32 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 24-53 in the N-terminal region of human IL32, UniProt Accession #P24001; NP_001012650.1; NP_001012649.1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in SK-BR-3 cell line lysates (35ug/lane) usingMBS649655. This demonstrates that MBS649655 detected the IL32 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue using MBS649655 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS649655 for immunohistochemistry.)

Related Product Information for anti-IL32 antibody

This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product Categories/Family for anti-IL32 antibody

Antibodies; Growth Factors; Cytokines

References

1. Li, W., et al. J. Immunol. 185(9):5056-5065(2010). 2. Cho, K.A., et al. Microb. Pathog. 49(3):95-104(2010). 3. Kobori, A., et al. World J. Gastroenterol. 16(19):2355-2361(2010). 4. Yue, D., et al. Asian J. Androl. 12(3):381-389(2010). 5. Chae, J.I., et al. Cell. Immunol. 264(1):47-53(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 61658632
• NCBI GeneID: 9235
• NCBI Accession #: NP_001012650.1; NP_001012649.1
• NCBI GenBank Nucleotide #: NP_001012650.1
• UniProt Accession #: P24001
• NCBI Official Full Name: interleukin-32 isoform B
• NCBI Official Synonym Full Names: interleukin 32
• NCBI Official Symbol: IL32
• NCBI Official Synonym Symbols: NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
• NCBI Protein Information: interleukin-32
• UniProt Protein Name: Interleukin-32
• Protein Family: Interleukin
• UniProt Gene Name: IL32
• UniProt Synonym Gene Names: NK4; TAIF; IL-32
• UniProt Entry Name: IL32_HUMAN

NCBI Description

This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

IL32: Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF- alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular space; membrane

Molecular Function: protein binding; cytokine activity

Biological Process: defense response; immune response; cell adhesion