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Western Blot (WB) (Western Blot analysis in SK-BR-3 cell line lysates (35ug/lane) using MBS649655. This demonstrates that MBS649655 detected the IL32 protein (arrow).)

Rabbit anti-Human IL32 Polyclonal Antibody | anti-IL32 antibody

IL32, NT (IL32, NK4, TAIF, Interleukin-32, Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor)

Gene Names
IL32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
IL32; Polyclonal Antibody; NT (IL32; NK4; TAIF; Interleukin-32; Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor); Anti -IL32; anti-IL32 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-IL32 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunogen
IL32 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-53 amino acids from the N-terminal region of human IL32.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in SK-BR-3 cell line lysates (35ug/lane) using MBS649655. This demonstrates that MBS649655 detected the IL32 protein (arrow).)

Western Blot (WB) (Western Blot analysis in SK-BR-3 cell line lysates (35ug/lane) using MBS649655. This demonstrates that MBS649655 detected the IL32 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue using MBS649655 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS649655 for immunohistochemistry.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue using MBS649655 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS649655 for immunohistochemistry.)
Related Product Information for anti-IL32 antibody
This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Product Categories/Family for anti-IL32 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,676 Da
NCBI Official Full Name
interleukin-32 isoform B
NCBI Official Synonym Full Names
interleukin 32
NCBI Official Symbol
IL32
NCBI Official Synonym Symbols
NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
NCBI Protein Information
interleukin-32; interleukin-32 eta; interleukin-32 small; interleukin-32 theta; natural killer cells protein 4; natural killer cell transcript 4; tumor necrosis factor alpha-inducing factor
UniProt Protein Name
Interleukin-32
Protein Family
UniProt Gene Name
IL32
UniProt Synonym Gene Names
NK4; TAIF; IL-32
UniProt Entry Name
IL32_HUMAN

NCBI Description

This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. Ref.2

Subcellular location: Secreted Ref.2.

Tissue specificity: Selectively expressed in lymphocytes. Expression is more prominent in immune cells than in non-immune cells. Ref.2

Induction: Expression increased after activation of T-cells by mitogens or activation of NK cells by IL2/interleukin-2. Ref.1

Research Articles on IL32

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Product Notes

The IL32 il32 (Catalog #AAA649655) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL32, NT (IL32, NK4, TAIF, Interleukin-32, Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL32 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the IL32 il32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL32, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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