Mouse Bone Morphogenetic Protein 2 ELISA Kit | BMP-2 elisa kit

Mouse Bone Morphogenetic Protein 2 ELISA Kit

Cat. Num. AAA763477

• Gene Names: BMP2; BDA2; BMP2A
• Reactivity: Mouse
• Synonyms: Bone Morphogenetic Protein 2; Mouse Bone Morphogenetic Protein 2 ELISA Kit; BMP-2 (Bone Morphogenetic Protein 2)/BMP2A/BMP-2A; BMP-2 elisa kit

• Reactivity: Mouse
• Sequence Length: 396

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 62.5-4000pg/ml
• Sensitivity: 37.5pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for BMP-2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse BMP-2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse BMP-2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse BMP-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse BMP-2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4557369
• NCBI GeneID: 650
• NCBI Accession #: NP_001191.1
• NCBI GenBank Nucleotide #: NM_001200.3
• Molecular Weight: 44,702 Da
• NCBI Official Full Name: bone morphogenetic protein 2 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 2
• NCBI Official Symbol: BMP2
• NCBI Official Synonym Symbols: BDA2; BMP2A
• NCBI Protein Information: bone morphogenetic protein 2
• UniProt Protein Name: Bone morphogenetic protein 2
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP2
• UniProt Synonym Gene Names: BMP2A; BMP-2; BMP-2A
• UniProt Entry Name: BMP2_HUMAN

NCBI Description

This gene encodes a member of the transforming growth factor-beta (TGFB) superfamily. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which induces bone and cartilage formation. [provided by RefSeq, Nov 2015]

Uniprot Description

Induces cartilage and bone formation (PubMed:3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed:24362451).