Rabbit anti-Human IL1A Polyclonal Antibody | anti-IL1A antibody

IL1A Antibody

Cat. Num. AAA2519606

• Gene Names: IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: IL1A; Polyclonal Antibody; IL1A Antibody; IL-1A; IL1; IL1-ALPHA; IL1F1; anti-IL1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 271

• Applicable Applications for anti-IL1A antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Recombinant protein of human IL1A
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-12741 / sc-32814 / sc-7929
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using IL1A antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human lung cancer using IL1A antibody at dilution of 1:200 (400x lens).)

Related Product Information for anti-IL1A antibody

IL-1alpha is a pro-inflammatory cytokine produced by activated monocytes, lymphocytes and epithelial cells (1). IL-1alpha is synthesized as an active precursor protein that appears to be cleaved by cytosolic proteases into its mature form (1, 2). Often, precursor and mature forms of IL-1alpha are primarily retained intracellularly rather than constitutively secreted. (1, 2). Signaling by IL-1alpha involves IL-1alpha binding to an IL-1 accessory protein (IL-1-AcP) and then the complex binds to IL-1RI (1, 2). Signaling is through activation of MAP kinase and NFkappaB pathways (1, 2). IL-1alpha also binds to an IL-RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. Inhibition of IL-1alpha activity is through IL-1R antagonist (IL-1Ra) that binds IL-1R1 but does not signal. IL-1alpha has been shown to be a key mediator of virus-induced inflammatory responses in mice (3).

NCBI and Uniprot Product Information

• NCBI GI #: 48146945
• NCBI GeneID: 3552
• UniProt Accession #: P01583; Q53QF9; Q7RU02
• Molecular Weight: 30,607 Da
• NCBI Official Full Name: IL1A
• NCBI Official Synonym Full Names: interleukin 1, alpha
• NCBI Official Symbol: IL1A
• NCBI Official Synonym Symbols: IL1; IL-1A; IL1F1; IL1-ALPHA
• NCBI Protein Information: interleukin-1 alpha; IL-1 alpha; hematopoietin-1; preinterleukin 1 alpha; pro-interleukin-1-alpha
• UniProt Protein Name: Interleukin-1 alpha
• Protein Family: Interleukin
• UniProt Gene Name: IL1A
• UniProt Synonym Gene Names: IL1F1; IL-1 alpha
• UniProt Entry Name: IL1A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]

Uniprot Description

IL1A: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family.

Protein type: Cytokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function: protein binding; copper ion binding; interleukin-1 receptor binding; cytokine activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; apoptosis; positive regulation of interleukin-2 biosynthetic process; cytokine and chemokine mediated signaling pathway; germ cell programmed cell death; positive regulation of JNK cascade; fever; cell proliferation; negative regulation of cell proliferation; positive regulation of angiogenesis; connective tissue replacement during inflammatory response; response to copper ion; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response; positive regulation of cytokine secretion