Rabbit IL1A Polyclonal Antibody | anti-IL1A antibody

IL1A Antibody

Cat. Num. AAA9607546

• Gene Names: IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Peptide affinity purification
• Synonyms: IL1A; Polyclonal Antibody; IL1A Antibody; BAF; FAF; Hematopoietin 1; Hematopoietin-1; IL 1 alpha; IL 1A; IL-1 alpha; Il-1a; IL1 ALPHA; IL1; IL1A_HUMAN; IL1F1; Interleukin 1 alpha; Interleukin-1 alpha; Interleukin1 alpha; LAF; LEM; Preinterleukin 1 alpha; Pro interleukin 1 alpha; anti-IL1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IL1 alpha antibody detects endogenous levels of total IL1 alpha
• Purity/Purification: Peptide affinity purification
• Form/Format: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: ~1mg/ml (varies by lot)
• Sequence Length: 271

• Applicable Applications for anti-IL1A antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; EIA(peptide): 1:20000-1:40000; ; IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween^®20 at 4°C with gentle shaking, overnight.
• Immunogen: A synthesized peptide derived from human IL1 alpha, corresponding to a region within N-terminal amino acids.
• Notes: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
• Preparation and Storage: Store at -20°C.

Western Blot (WB)

(Western blot analysis of extracts from mouse brain, using IL1A Antibody.)

Immunohistochemistry (IHC)

(MBS9607546 at 1/100 staining Human uterus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-IL1A antibody

IL-1 alpha is a pro-inflammatory cytokine produced by activated monocytes, lymphocytes and epithelial cells (1). IL-1 alpha is synthesized as an active precursor protein that appears tobe cleaved by cytosolic proteases into its mature form (1,2). Often, precursor and mature forms of IL-1 alpha are primarily retained intra cellularly rather than constitutively secreted. (1,2). Signaling by IL-1 alpha involves IL-1 alpha binding to an IL-1 accessory protein (IL-1-AcP) and then the complex binds toIL-1RI (1,2). Signaling is through activation of MAP kinaseand NFalphaB pathways (1,2). IL-1 alpha also binds to an IL-RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. Inhibition of IL-1 alpha activity is through IL-1R antagonist (IL-1Ra) that binds IL-1R1 but does not signal.

NCBI and Uniprot Product Information

• NCBI GI #: 27894330
• NCBI GeneID: 3552
• NCBI Accession #: NP_000566.3
• NCBI GenBank Nucleotide #: NM_000575.4
• UniProt Accession #: P01583; Q53QF9; Q7RU02
• Molecular Weight: 31 kDa
• NCBI Official Full Name: interleukin-1 alpha
• NCBI Official Synonym Full Names: interleukin 1 alpha
• NCBI Official Symbol: IL1A
• NCBI Official Synonym Symbols: IL1; IL-1A; IL1F1; IL1-ALPHA
• NCBI Protein Information: interleukin-1 alpha
• UniProt Protein Name: Interleukin-1 alpha
• Protein Family: Interleukin
• UniProt Gene Name: IL1A
• UniProt Synonym Gene Names: IL1F1; IL-1 alpha

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]

Uniprot Description

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.