Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using IL1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Rabbit anti-Human, Mouse IL1A Polyclonal Antibody | anti-IL1A antibody
IL1A Polyclonal Antibody
Gene Names
IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity purification
Synonyms
IL1A; Polyclonal Antibody; IL1A Polyclonal Antibody; IL1; IL1ALPHA; IL1F1; Interleukin1 alpha; Hematopoietin1;; anti-IL1A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
271
Applicable Applications for anti-IL1A antibody
Western Blot (WB)
Application Notes
Western Blot (WB): 1:500-1:2000
Immunogen
A synthetic peptide of human IL1A
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C or -80 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using IL1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using IL1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human esophagus using IL1A antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human esophagus using IL1A antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse brain using IL1A antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse brain using IL1A antibody at dilution of 1:100 (40x lens).)
Related Product Information for anti-IL1A antibody
IL1alpha is a proinflammatory cytokine produced by activated monocytes, lymphocytes, and epithelial cells (1). IL1alpha is synthesized as an active precursor protein that appears to be cleaved by cytosolic proteases into its mature form (1,2). Often, precursor and mature forms of IL1alpha are primarily retained intracellularly rather than constitutively secreted. (1,2). Signaling by IL1alpha involves IL1alpha binding to an IL1 accessory protein (IL1AcP) and then the complex binds to IL1RI (1,2). Signaling occurs through activation of MAP kinase and NFkappaB pathways (1,2). IL1alpha also binds to an ILRII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. IL1alpha activity is inhibited through IL1R antagonist (IL1Ra) that binds IL1R1 but does not signal. IL1alpha has been shown to be a key mediator of virusinduced inflammatory responses in mice (3).
References
1. Dinarello, C.A. (1996) Blood 87, 2095147 2. Allan, S.M. et al. (2005) Nat Rev Immunol 5, 62940. 3. Di Paolo, N.C. et al. (2009) Immunity 31, 11021
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17kDa
NCBI Official Full Name
interleukin-1 alpha proprotein
NCBI Official Synonym Full Names
interleukin 1, alpha
NCBI Official Symbol
IL1A
NCBI Official Synonym Symbols
IL1; IL-1A; IL1F1; IL1-ALPHA
NCBI Protein Information
interleukin-1 alpha; IL-1 alpha; hematopoietin-1; preinterleukin 1 alpha; pro-interleukin-1-alpha
UniProt Protein Name
Interleukin-1 alpha
Protein Family
UniProt Gene Name
IL1A
UniProt Synonym Gene Names
IL1F1; IL-1 alpha
UniProt Entry Name
IL1A_HUMAN
Similar Products
Product Notes
The IL1A il1a (Catalog #AAA126562) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL1A Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot (WB): 1:500-1:2000. Researchers should empirically determine the suitability of the IL1A il1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
