Interleukin 1 Beta (IL1b) Recombinant Protein | IL1b recombinant protein
Recombinant Interleukin 1 Beta (IL1b)
Gene Names
IL1B; IL-1
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Interleukin 1 Beta (IL1b); Recombinant Interleukin 1 Beta (IL1b); IL1b recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGS-CKTDADNF MSDAAMQSVD CKLQDISHKY LVLSNSYELR ALHLNGENVN KQVVFHMSFV HGDESNNKIP VVLGIKQKNL YLSCVMKDGK PTLQLEKVDP KVYPKRKMEK RFVFNKIEIK NTVEFESSQY PNWYISTSQV EGMPVFLGNT RGGQDITDFT
MGHHHHHHSGS-CKTDADNF MSDAAMQSVD CKLQDISHKY LVLSNSYELR ALHLNGENVN KQVVFHMSFV HGDESNNKIP VVLGIKQKNL YLSCVMKDGK PTLQLEKVDP KVYPKRKMEK RFVFNKIEIK NTVEFESSQY PNWYISTSQV EGMPVFLGNT RGGQDITDFT
Sequence Length
265
Applicable Applications for IL1b recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Canis familiaris; Canine (Dog)
Expression System
Prokaryotic expression
Residues
Cys103~Thr260 (Accession # Q2VCE2) with N-terminal His-Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19.3kDa
NCBI Official Full Name
interleukin-1 beta
NCBI Official Symbol
IL1B
NCBI Official Synonym Symbols
IL-1
NCBI Protein Information
interleukin-1 beta; IL-1 beta; interleukin-1 beta proprotein
UniProt Protein Name
Interleukin-1 beta
Protein Family
UniProt Gene Name
IL1B
UniProt Entry Name
Q2VCE2_CANFA
Uniprot Description
Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells
By similarity. RuleBase RU003753
Subunit structure: Monomer
By similarity. RuleBase RU003753
Sequence similarities: Belongs to the IL-1 family. RuleBase RU003752
