Western Blot (WB)
(Anti-IL-8 antibody, MBS175692, Western blottingLane 1: Recombinant Human IL-18 Protein 10ngLane 2: Recombinant Human IL-18 Protein 5ngLane 3: Recombinant Human IL-18 Protein 2.5ng )
Rabbit anti-Human IL-8 Polyclonal Antibody | anti-IL8 antibody
Anti-IL-8 antibody
Concentration: 0.1-0.5ug/ml
Tested Species: Hu
Predicted Species: -
Antigen Retrieval: -
Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Hu
Predicted Species: -
Antigen Retrieval: By Heat
Tested Species: In-house tested species with positive results.
Predicted Species: Species prediceted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Western Blot (WB)
(Anti-IL-8 antibody, MBS175692, Western blottingLane 1: Recombinant Human IL-18 Protein 10ngLane 2: Recombinant Human IL-18 Protein 5ngLane 3: Recombinant Human IL-18 Protein 2.5ng )
Western Blot (WB)
(Anti-IL-8 antibody, MBS175692, Western blottingLane 1: Recombinant Human IL-18 Protein 10ngLane 2: Recombinant Human IL-18 Protein 5ngLane 3: Recombinant Human IL-18 Protein 2.5ng )
Immunohistochemistry (IHC)
(Anti-IL-8 antibody, MBS175692, IHC(P)IHC(P): Human Appendicitis Tissue )
Immunohistochemistry (IHC)
(Anti-IL-8 antibody, MBS175692, IHC(P)IHC(P): Human Appendicitis Tissue )
Background: Interleukin-8(IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. In humans, the interleukin-8 protein is encoded by the IL8 gene. Interleukin-8(IL8) is a member of the CXC chemokine family(Hull et al., 2001). IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. The genes for IL8 have been co-localized on a 335-kb genomic fragment.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008]
Uniprot Description
IL8: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. Belongs to the intercrine alpha (chemokine CxC) family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Cytokine; Secreted; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 4q13-q21
Cellular Component: extracellular space; extracellular region
Molecular Function: protein binding; chemokine activity; interleukin-8 receptor binding
Biological Process: regulation of cell adhesion; neutrophil chemotaxis; neutrophil activation; negative regulation of G-protein coupled receptor protein signaling pathway; unfolded protein response; calcium-mediated signaling; chemotaxis; signal transduction; regulation of retroviral genome replication; induction of positive chemotaxis; G-protein coupled receptor protein signaling pathway; negative regulation of cell proliferation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; response to molecule of bacterial origin; receptor internalization; immune response; angiogenesis; cell cycle arrest; cell motility; inflammatory response; embryonic gut development
Research Articles on IL8
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Product Notes
The IL8 cxcl8 (Catalog #AAA175692) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL-8 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot (WB): Concentration: 0.1-0.5ug/ml Tested Species: Hu Predicted Species: - Antigen Retrieval: - Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1ug/ml Tested Species: Hu Predicted Species: - Antigen Retrieval: By Heat Tested Species: In-house tested species with positive results. Predicted Species: Species prediceted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the IL8 cxcl8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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