Rabbit anti-Human, Mouse COLQ Polyclonal Antibody | anti-COLQ antibody

COLQ, CT (COLQ, Acetylcholinesterase collagenic tail peptide, AChE Q subunit, Acetylcholinesterase-associated collagen) (APC)

Cat. Num. AAA6287780

• Gene Names: COLQ; EAD; CMS5
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: COLQ; Polyclonal Antibody; CT (COLQ; Acetylcholinesterase collagenic tail peptide; AChE Q subunit; Acetylcholinesterase-associated collagen) (APC); Acetylcholinesterase-associated collagen; anti-COLQ antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-COLQ antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: COLQ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 324-353 amino acids from the C-terminal region of human COLQ.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(COLQ Antibody (C-term) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the COLQ antibody detected the COLQ protein (arrow).)

Related Product Information for anti-COLQ antibody

This gene encodes the subunit of a collagen-like molecule associated with acetylcholinesterase in skeletal muscle. Each molecule is composed of three identical subunits. Each subunit contains a proline-rich attachment domain (PRAD) that binds an acetylcholinesterase tetramer to anchor the catalytic subunit of the enzyme to the basal lamina. Mutations in this gene are associated with endplate acetylcholinesterase deficiency. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-COLQ antibody

Antibodies; Enzymes; Acetylcholinesterase

References

Mihaylova, V., et al. Brain 131 (PT 3), 747-759 (2008): Schreiner, F., et al. Neuromuscul. Disord. 17(3):262-265(2007). Ting, A.K., et al. Chem. Biol. Interact. 157-158, 63-70 (2005): Dvir, H., et al. EMBO J. 23(22):4394-4405(2004). Ishigaki, K., et al. Neuromuscul. Disord. 13(3):236-244(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 145701009
• NCBI GeneID: 8292
• NCBI Accession #: NP_536800.2
• NCBI GenBank Nucleotide #: NM_080539.3
• UniProt Accession #: Q9Y215
• Molecular Weight: 421
• NCBI Official Full Name: acetylcholinesterase collagenic tail peptide isoform III
• NCBI Official Synonym Full Names: collagen like tail subunit of asymmetric acetylcholinesterase
• NCBI Official Symbol: COLQ
• NCBI Official Synonym Symbols: EAD; CMS5
• NCBI Protein Information: acetylcholinesterase collagenic tail peptide
• UniProt Protein Name: Acetylcholinesterase collagenic tail peptide
• UniProt Gene Name: COLQ
• UniProt Entry Name: COLQ_HUMAN

NCBI Description

This gene encodes the subunit of a collagen-like molecule associated with acetylcholinesterase in skeletal muscle. Each molecule is composed of three identical subunits. Each subunit contains a proline-rich attachment domain (PRAD) that binds an acetylcholinesterase tetramer to anchor the catalytic subunit of the enzyme to the basal lamina. Mutations in this gene are associated with endplate acetylcholinesterase deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

COLQ: Anchors the catalytic subunits of asymmetric AChE to the synaptic basal lamina. Defects in COLQ are the cause of congenital myasthenic syndrome Engel type (CMSE); also known as end-plate acetylcholinesterase deficiency or congenital myasthenic syndrome type IC (CMS-IC). CMSE is a rare autosomal recessive congenital myasthenic syndrome characterized by onset during childhood, generalized weakness, abnormal fatigability on exertion, refrectoriness to acetylcholinesterase drugs, decremental electromyographic response and morphological abnormalities of the neuromuscular junctions. Belongs to the COLQ family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: extracellular space; collagen; synapse; basal lamina; cell junction

Molecular Function: protein binding

Biological Process: asymmetric protein localization; acetylcholine catabolic process in synaptic cleft

Disease: Endplate Acetylcholinesterase Deficiency