Rabbit anti-Human IL-18 Polyclonal Antibody | anti-IL18 antibody

Anti-IL-18 antibody

Cat. Num. AAA175566

• Gene Names: IL18; IGIF; IL-18; IL-1g; IL1F4
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: IL-18; Polyclonal Antibody; Anti-IL-18 antibody; anti-IL18 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 193

• Applicable Applications for anti-IL18 antibody: Western Blot (WB)
• Application Notes: WB - Concentration: 0.1-0.5ug/ml; Tested Species: Human (In-house tested species with positive results.)
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of Human IL18 (175-193aa KKEDELGDRSIMFTVQNED).
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-IL-18 antibody, Western blottingLane 1: Recombinant Human IL-18 Protein 10ngLane 2: Recombinant Human IL-18 Protein 5ngLane 3: Recombinant Human IL-18 Protein 2.5ng Lane 4: RAJI Cell Lysate)

Western Blot (WB)

(Western blot analysis of IL-18 expression in HELA whole cell lysates (lane 1). IL-18 at 22KD was detected using rabbit anti- IL-18 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.)

Related Product Information for anti-IL18 antibody

Interleukin-18 also known as IL18 is a protein which in humans is encoded by the IL18 gene. The protein encoded by this gene is a proinflammatory cytokine.IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide(LPS). After stimulation with IL-18, natural killer(NK) cells and certain T cells release another important cytokine called interferon-gamma(IFN-gamma) or type II interferon that plays an important role in activating the macrophages or other cells.The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein(IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. The human interleukin 18 gene IL18 maps to 11q22.2-q22.3, closely linked to the DRD2 gene locus and distinct from mapped IDDM loci.

NCBI and Uniprot Product Information

• NCBI GI #: 3219817
• NCBI GeneID: 3606
• UniProt Accession #: Q14116; O75599; Q6FGY3; Q6WWJ7
• NCBI Official Full Name: Interleukin-18
• NCBI Official Synonym Full Names: interleukin 18
• NCBI Official Symbol: IL18
• NCBI Official Synonym Symbols: IGIF; IL-18; IL-1g; IL1F4
• NCBI Protein Information: interleukin-18; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)
• UniProt Protein Name: Interleukin-18
• Protein Family: Interleukin
• UniProt Gene Name: IL18
• UniProt Synonym Gene Names: IGIF; IL1F4; IL-18; IFN-gamma-inducing factor; IL-1 gamma
• UniProt Entry Name: IL18_HUMAN

NCBI Description

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

Uniprot Description

IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 11q22.2-q22.3

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function: protein binding; cytokine activity

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of NK T cell proliferation; natural killer cell activation; positive regulation of NF-kappaB import into nucleus; positive regulation of activated T cell proliferation; chemokine biosynthetic process; cell-cell signaling; lipopolysaccharide-mediated signaling pathway; granulocyte macrophage colony-stimulating factor biosynthetic process; angiogenesis; inflammatory response; regulation of cell adhesion; positive regulation of interleukin-17 production; positive regulation of natural killer cell proliferation; T-helper 1 type immune response; interleukin-2 biosynthetic process; T-helper 2 type immune response; MAPKKK cascade; interleukin-13 biosynthetic process; sleep; positive regulation of tissue remodeling; positive regulation of interferon-gamma production; interferon-gamma biosynthetic process; immune response; negative regulation of myoblast differentiation; positive regulation of inflammatory response