Immunohistochemistry (IHC)
(Anti-IL-18 Picoband antibody, MBS176767-1.JPGIHC(P): Human Tonsil Tissue )
Rabbit anti-Human IL18 Polyclonal Antibody | anti-IL18 antibody
Anti-IL18 antibody
Concentration: 0.1-0.5ug/ml
Tested Species: Hu
Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Hu
Antigen Retrieval: By Heat
Immunocytochemistry:
Concentration: 0.58-1ug/ml
Tested Species: Hu
WB: The detection limit for IL18 is approximately 0.25ng/lane under reducing conditions.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions shoudl be determined by end users.
Immunohistochemistry (IHC)
(Anti-IL-18 Picoband antibody, MBS176767-1.JPGIHC(P): Human Tonsil Tissue )
Immunohistochemistry (IHC)
(Anti-IL-18 Picoband antibody, MBS176767-1.JPGIHC(P): Human Tonsil Tissue )
Western Blot (WB)
(Anti-IL-18 Picoband antibody, MBS176767-2.jpgAll lanes: Anti-IL18 (MBS176767) at 0.5ug/mlWB: HELA Whole Cell Lysate at 40ugPredicted bind size: 22KDObserved bind size: 22KD )
Western Blot (WB)
(Anti-IL-18 Picoband antibody, MBS176767-2.jpgAll lanes: Anti-IL18 (MBS176767) at 0.5ug/mlWB: HELA Whole Cell Lysate at 40ugPredicted bind size: 22KDObserved bind size: 22KD )
Background: Interleukin-18 also known as IL18 is a protein which in humans is encoded by the IL18 gene. The protein encoded by this gene is a proinflammatory cytokine.IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-gamma (IFN-gamma) or type II interferon that plays an important role in activating the macrophages or other cells.The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. The human interleukin 18 gene IL18 maps to 11q22.2-q22.3, closely linked to the DRD2 gene locus and distinct from mapped IDDM loci.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
Uniprot Description
IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family.
Protein type: Cytokine
Chromosomal Location of Human Ortholog: 11q22.2-q22.3
Cellular Component: extracellular space; extracellular region; cytosol
Molecular Function: protein binding; cytokine activity
Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of interleukin-17 production; regulation of cell adhesion; T-helper 1 type immune response; positive regulation of natural killer cell proliferation; interleukin-2 biosynthetic process; T-helper 2 type immune response; positive regulation of NK T cell proliferation; MAPKKK cascade; natural killer cell activation; interleukin-13 biosynthetic process; positive regulation of NF-kappaB import into nucleus; positive regulation of activated T cell proliferation; sleep; chemokine biosynthetic process; positive regulation of tissue remodeling; positive regulation of interferon-gamma production; cell-cell signaling; lipopolysaccharide-mediated signaling pathway; interferon-gamma biosynthetic process; granulocyte macrophage colony-stimulating factor biosynthetic process; immune response; angiogenesis; inflammatory response; negative regulation of myoblast differentiation; positive regulation of inflammatory response
Research Articles on IL18
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Product Notes
The IL18 il18 (Catalog #AAA176767) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL18 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL18 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot: Concentration: 0.1-0.5ug/ml Tested Species: Hu Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1ug/ml Tested Species: Hu Antigen Retrieval: By Heat Immunocytochemistry: Concentration: 0.58-1ug/ml Tested Species: Hu WB: The detection limit for IL18 is approximately 0.25ng/lane under reducing conditions. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions shoudl be determined by end users. Researchers should empirically determine the suitability of the IL18 il18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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